추천 제품
양식
buffered aqueous solution
분자량
size 6185 bp
박테리아 선정
kanamycin
포유류 세포 선정
puromycin
복제개시점
pUC (500 copies)
펩타이드 절단
no cleavage
펩타이드 태그 위치
N-terminal
촉진제
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
리포터 유전자
none
배출 신호
human IL-2
배송 상태
ambient
저장 온도
−20°C
일반 설명
This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains an Human Interleukin-2 (IL-2) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
About the Peptide Tag:This plasmid contains an Human Interleukin-2 (IL-2) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
서열
To view sequence information for this product, please visit the product page
분석 메모
To view the Certificate of Analysis for this product, please visit www.oxgene.com
관련 제품
제품 번호
설명
가격
Storage Class Code
12 - Non Combustible Liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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활성 필터
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