There are no definitive references or citations confirming the light sensitivity of this material. However, this product is packaged in amber glass may be susceptible to light, particularly at UV wavelengths. In bright field microscopy slides must be viewed in subdued light. While this item is not tested for use in fluorescent, it has been used on Flow Cytometry and has an emission wavelength of approximately 600 nm. There are a number of publication indicating light sensitivity. See the link below to review on such example:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931440/
추천 제품
양식
liquid
농도
0.5% in isopropanol
기술
microbe id | staining: suitable
색상
dark red
εmax
0.20-0.50 at 510-517 nm
응용 분야
diagnostic assay manufacturing
hematology
histology
저장 온도
room temp
SMILES string
N(\N=C3\c4c(cccc4)C=CC\3=O)c1c(cc(c(c1)C)N=Nc2c(ccc(c2)C)C)C
InChI
1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-
InChI key
HJAYEODIXUYVIC-LTFAAIOASA-N
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
애플리케이션
Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
표적 기관
Central nervous system
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
Flash Point (°F)
53.6 °F - closed cup
Flash Point (°C)
12.0 °C - closed cup
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
LWW Doody's all reviewed collection, McClatchey: Clinical Laboratory Medicine
Clinical Laboratory Medicine (2002)
Hepatic Peroxisome Proliferator-Activated Receptor Gamma Signaling Contributes to Alcohol-Induced Hepatic Steatosis and Inflammation in Mice.
Zhang W, et al.
Alcoholism, Clinical and Experimental Research, 40(5), 988-999 (2016)
Sulforaphane inhibits mammary adipogenesis by targeting adipose mesenchymal stem cells.
Li Q, et al.
Breast Cancer Research and Treatment, 141(2), 317-324 (2013)
Pablo Sanchez Bosch et al.
Developmental cell, 51(6), 787-803 (2019-11-19)
The use of adult Drosophila melanogaster as a model for hematopoiesis or organismal immunity has been debated. Addressing this question, we identify an extensive reservoir of blood cells (hemocytes) at the respiratory epithelia (tracheal air sacs) of the thorax and
Tommy Weiss-Sadan et al.
Theranostics, 9(20), 5731-5738 (2019-09-20)
Despite the common use of lipid-lowering medications, cardiovascular diseases continue to be a significant health concern. Atherosclerosis, one of the most frequent causes of cardiovascular morbidity, involves extensive inflammatory activity and remodeling of the vascular endothelium. This relentless inflammatory condition
문서
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.
Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.
프로토콜
Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.
Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.
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Hi! I'm wondering if OilRedO is light sensitive? Does the incubation need to take place in the dark? And could the staining of cells be compromized if the solution is not kept dark?
1 답변-
도움이 되었습니까?
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What are the differences between Oil Red O solution O1391 and OIL RED O staining solution 1.02419?
1 답변-
There are not many differences between products O1391 and 102419. O1391 is available in 250 mL and 500 mL bottles, while 102419 is available only in a 250 ml bottle. The only practical difference between the two products is the solvents used to dissolve the Oil Red O. For O1391, the solvent used is isopropyl alcohol, while the solvents used for 102419 are acetone and ethyl alcohol. Both products use Oil Red O as the dye, and the directions for use call for diluting the product with water prior to use and then filtering. The results should be identical since both products use Oil Red O as the dye.
도움이 되었습니까?
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Should cells or tissues be fixed before staining with Oil Red O for fats or lipids?
1 답변-
Yes, cells or tissues need to be fixed before staining with Oil Red O. It is important to avoid using fixatives that contain alcohol. Oil Red O staining can be performed on cultured cells and fresh frozen tissue. However, tissues should not be paraffin embedded and exposed to the alcohols and solvents necessary for paraffin processing tissue.
도움이 되었습니까?
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Hi I purchased Oil Red O solution – 0.5% in isopropanol, 01391 250mL, PCode-1003585017, Source, SLCP2956 for staining of whole mice aorta. I want to dilute it to 0.16% solution. What solution should I use for dilution? MilliQ water/methanol/isopropanol?
1 답변-
This product can be further diluted using distilled water or aqueous buffer to reach the desired concentration. Note that solution diluted with water or aqueous buffers are less stable and should not be stored for extended periods.
도움이 되었습니까?
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Can I use this solution for stain frozen sections ? I thought that I bought oil red in propylene glycol. What is the difference ?
1 답변-
This O1391 is a 0.5% solution in isopropanol. Product O1516 is a 0.5% solution in propylene glycol. The protocol for either product is the same. There is some evidence in the literature that the propylene glycol solution performs better on PFPP samples, however this is not definitive and may depend on conditions and technique. Each is considered a stock concentration. Stock solutions can range from 0.2 - 0.5%. Depending on the specific application the stock solution will need to be further diluted by 40 - 60%. For example, in staining frozen sections, 30ml of the O1391 would be diluted with 20ml of distilled water to reach a working concentration. Note that solution diluted with water or aqueous buffers are less stable and should not be stored for extended periods.
Please see the link below to review and article that may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449473/pdf/10616_2004_Article_3903.pdf도움이 되었습니까?
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How do you prepare the working solution using this reagent? or is it already a prepared working solution?
1 답변-
This product is a 0.5% solution in isopropanol and is considered a stock concentration. Stock solution can range from 0.2 - 0.5%. Depending on the specific application the stock solution will need to be further diluted by 40 - 60%. For example, in staining frozen sections, 30ml of the O1391 would be diluted with 20ml of distilled water to reach a working concentration. Note that solution diluted with water or aqueous buffers are less stable and should not be stored for extended periods.
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