MTOX1005

MDR1/MRP2 Double Knockout Caco-2 Cells

Human male colorectal tissue, adenocarcinoma

• 동의어(들): C2BBe1 Cells MDR1/MRP2 (-/-/-/-,-/-/-/-)
• UNSPSC 코드: 41106514
• NACRES: NA.81

속성

• 제품명: MDR1/MRP2 Double Knockout Caco-2 Cells, one vial
• 생물학적 소스: human male colorectal tissue (Source disease: adenocarcinoma)
• 양식: liquid
• 기술: drug transporter assay: suitable; permeability assay: suitable
• OMIM 수납 번호: 171050; 601107
• 응용 분야: ADME/TOX
• 저장 온도: −196°C
• 유전자 정보: human ... ABCB1(5243) , ABCC2(1244)

설명

일반 설명

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 and MRP2 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional double knockout of the ABCB1 and ABCC2 (MDR1/MRP2) efflux transporters.

애플리케이션

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

특징 및 장점

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:

• A functional double knockout of the MDR1 gene and MRP2 gene eliminates the reliance on chemical inhibitors to determine if a compound is an BCRP substrate
• The vial format enables the MDR1 and MRP2 knockout cells to be included in standard drug transporter protocols
• Human assay with no interference from animal inhibitors
• Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

법적 정보

ADME/Tox Cell Lines License

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

키트 구성품 역시 별도로 이용 가능함

• MTOX1005 MDR1/MRP2 Double Knockout Caco-2 Cells, one vial 1 vial

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable