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Merck
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Key Documents

MAK364

Sigma-Aldrich

Reduced Glutathione (GSH) Assay Kit (Colorimetric)

sufficient for 100 colorimetric tests

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric tests

검출 방법

colorimetric

관련 질환(들)

cancer

저장 온도

−20°C

일반 설명

The new Glutathione (GSH) Assay Kit, MAK517, is now available! Glutathione (GSH), a thiol-containing tripeptide (γ-glutamyl-cysteinyl-glycine), is a key antioxidant in many species. It has been highly implicated in the detoxification/elimination of xenobiotics (naturally occurring harmful compounds such as free radicals, hydroperoxides etc.) and in the maintenance of the oxidation state of protein sulfhydryl groups. In addition, GSH plays a pivotal role in the pathogenesis of numerous human diseases including cancer and cardio-vascular diseases. Glutathione is present in cells in both reduced (GSH) and oxidized (GSSG) forms- GSH being, the predominant species under normal physiological conditions inside cells. Thus, pathologic conditions causing oxidative stress would result in increased levels of GSSG. Therefore, the measurement of intracellular GSH appears to be a sensitive indicator of the overall cell health, and its ability of resisting toxic challenges.

애플리케이션

<ul>
<li><strong>Hyperbaric oxygen protects HT22 cells and PC12 cells from damage caused by oxygen-glucose deprivation/reperfusion via the inhibition of Nrf2/System Xc-/GPX4 axis-mediated ferroptosis:</strong> This research uses the kit to monitor glutathione levels as part of a strategy to understand cell viability under stress, highlighting its utility in studies involving cellular defense mechanisms against oxidative damage (Chen et al., 2022).</li>
</ul>

적합성

The kit is suitable for the measurement of reduced glutathione in various biological samples/preparations including tissue homogenates (liver, etc.) and cell lysates (Hep G2, Jurkat, etc.).

원리

The Reduced Glutathione (GSH) Assay Kit is based on an enzymatic cycling method in the presence of GSH and a chromophore. The reduction of the chromophore produces a stable product, which can be followed kinetically at A450 nm. Therefore, its absorbance is directly proportional to the amount of GSH in the sample. The kit is highly specific and sensitive because GSSG does not interfere with the assay. The kit includes 5-Sulfosalicylic acid (SSA) in order to protect samples′ GSH endogenous content. (SSA acts as a deproteinizing and antioxidant agent. The assay is simple, reproducible and can specifically detect as low as 50 pmol/well of reduced form of Glutathione (GSH) in a 100 μL reaction.

교체됨

픽토그램

Health hazardCorrosion

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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