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Merck
모든 사진(3)

문서

MAK339

Sigma-Aldrich

Lipopolysaccharide (LPS) Isolation Kit

sufficient for 10 isolation(s)

동의어(들):

LPS Extraction Kit

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About This Item

UNSPSC 코드:
12161503

사용

sufficient for 10 isolation(s)

응용 분야

sample preservation

저장 온도

−20°C

일반 설명

The outer membrane of gram-negative bacteria contains lipopolysaccharide (LPS), a low molecular weight carbohydrate with a molecular mass of 10-20 kDa. It is heterogeneous and composed of O antigen (a repeating glycan polymer), core oligosaccharide (which links the O antigen to Lipid A - the third component, and non-carbohydrate components such as phosphate and amino acids groups. Lipid A, has multiple fatty acids which serve to anchor LPS into the bacterial membrane allowing the O antigen and core oligosaccharide to protrude and contributes to a major part of the toxicity of gram-negative bacteria. Also known as endotoxin, when consumed by animals, LPS induces a strong inflammatory response and/or sepsis. 

적합성

Suitable for the isolation of Lipopolysaccharides from outer membrane of gram negative bacteria.

원리

The Lipopolysaccharide (LPS) Isolation Kit uses bacterial membrane lysis buffer and protein digestion to yield micrograms of LPS from bacterial culture (approximately 1-4% of dry weight). This kit does not use chloroform or phenol like traditional methods, and it will yield pure LPS in less than 2 hours that can be easily characterized and quantified.

픽토그램

Health hazardCorrosion

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Met. Corr. 1 - Resp. Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Zhiyuan He et al.
Microbiome, 10(1), 79-79 (2022-06-02)
Antimicrobials are often used to prevent and treat diarrhea induced by enteroaggregative Escherichia coli (EAEC) in young ruminants. However, drug overuse or misuse accelerates the spread of multidrug-resistant extended-spectrum β-lactamase (ESBL)-producing E. coli. Thus, supplementary foods as alternatives to antibiotics
Zhiyuan He et al.
Frontiers in nutrition, 9, 864080-864080 (2022-04-12)
Calf diarrhea induced by enteroaggregative E. coli (EAEC) spreads fast among young ruminants, causing continuous hazard to dairy industry. Antimicrobial drug abuse aggravates the incidence rate of multi-drug resistant (MDR) extended-spectrum β-lactamase-producing E. coli (ESBL-EC). However, knowledge of detection and

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