콘텐츠로 건너뛰기
Merck
모든 사진(3)

주요 문서

L2880

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O55:B5

purified by phenol extraction

동의어(들):

LPS

로그인조직 및 계약 가격 보기

크기 선택

10 MG
₩176,712
25 MG
₩312,519
100 MG
₩725,015
500 MG
₩2,581,856

₩176,712

출고 가능일2025년 4월 10일세부사항

벌크 견적 요청
모든 사진(3)

크기 선택

보기 변경
10 MG
₩176,712
25 MG
₩312,519
100 MG
₩725,015
500 MG
₩2,581,856

About This Item

CAS Number:
93572-42-0
EC Number:
297-473-0
MDL number:
MFCD00164401
UNSPSC 코드:
12352201
NACRES:
NA.25

₩176,712

출고 가능일2025년 4월 10일세부사항

벌크 견적 요청

생물학적 소스

Escherichia coli (O55:B5)

Quality Level

300

양식

lyophilized powder

정제법

phenol extraction

기술

cell based assay: suitable

불순물

<3% Protein (Lowry)

색상

off-white to tan

solubility

water: soluble

응용 분야

cell analysis
clinical research
life science and biopharma

배송 상태

ambient

저장 온도

2-8°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

관련 카테고리

일반 설명

Lipopolysaccharides (LPS) are glycolipids,[1] a combination of carbohydrate units linked with single or multiple fatty acids that are found in the cell wall of Gram-negative bacteria.[2] The LPS is an essential component of the outer membrane and the LPS structure is composed of lipid A, a glucosamine-based phospholipid, a short-core oligosaccharide, and an O-antigen, a distal polysaccharide.[3]

애플리케이션

Lipopolysaccharide from Escherichia coli O55:B5 has been used:
  • to stimulate hepatocytes and non-parenchymal cells[4]
  • to stimulate the spleen and study the expression of the Malitaf gene in the fish blunt snout bream[5]
  • as part of the concanavalin A (ConA) and D-galactosamine/lipopolysaccharide (D-GalN/LPS) model of fulminant hepatitis to study the effect of the natural compound hesperetin[6]
  • to check the cell viability of human chorionic villi-derived mesenchymal stem cells (hCMSCs) by methyl thiazolyl tetrazolium (MTT) assay[7]
  • to induce oligodendrocyte toxicity to evaluate the neuroprotective effects of few compounds[8]
  • to induce sarcopenia in rats to evaluate the antioxidant effects of curcumin-loaded hydrophobic surface-modified hydroxyapatite[9]
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

생화학적/생리학적 작용

Lipopolysaccharides (LPS) sets up a protective permeability barrier that blocks the entry of antibiotics and bile salts, which make Gram-negative bacteria innately resistant. LPS is involved in bacterial pathogenicity as the host immune system primarily encounters the LPS component of the bacteria. LPS is an endotoxin and a highly immunogenic antigen that can enhance the immune system. The lipid A of LPS stimulates host cells via toll-like receptors 4 (TLR4).[1]

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
For additional information on our range of Biochemicals, please complete this form.
For optimal reconstitution of LPS products, an intensive vortex is required before use, regardless of the container type. Ensure that the storage solution concentration is at least 1 mg/ml. While borosilicate (silanized) containers are recommended, polypropylene (PP) plastic containers may also be used.
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

관련 제품

제품 번호
설명
가격

픽토그램

Skull and crossbones
GHS06

신호어

Danger

유해 및 위험 성명서

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000428249
0000428249
0000428255
0000428255
0000428250

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

A liver microphysiological system of tumor cell dormancy and inflammatory
responsiveness is affected by scaffold properties
Clark AM
Cancer Discovery null
Glycolipid biosurfactants: Biosynthesis and related potential applications in food industry
Ashby R D, et al.
Journal of Separation Science, 307-334 (2023)
The protective effect of the natural compound hesperetin against fulminant hepatitis in vivo and in vitro
Xueting Bai
British Journal of Pharmacology, 174 (2017)
Ya-Jyun Liang et al.
Antioxidants (Basel, Switzerland), 10(4) (2021-05-01)
Oxidative stress and later-induced chronic inflammation have been reported to play an important role on the progression of sarcopenia. Current treatments for sarcopenia are mainly administered to patients whom sarcopenia already developed. However, there has been no promising results shown
Identification and Characterization of Lipopolysaccharide Induced TNFa Factor from Blunt Snout Bream, Megalobrama amblycephala
Yina LV
International Journal of Molecular Sciences, 18(2), 233-233 (2017)
모든 관련된 서류 보기

문서

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

1개 상품평 이 작업을 수행하면 상품평으로 이동합니다.

질문

1–9 / 9 질문  

  1. I recently purchased this item and I would like to understand how lethal/toxic/dangerous is for humans once diluted to 0.5mg/ml. I would need to inject ip fish with a certain volume from that stock solution.

    1 답변

    1. Toxicological information, when available, is provided in section 11 of the safety data sheet. It is unclear which fish model is being used to study toxicity. Consulting scientific literature is recommended for these details. Below is some information that may be useful to address the question.

      https://www.sigmaaldrich.com/sds/sigma/l2880?userType=anonymous

      https://www.tandfonline.com/doi/abs/10.1080/10934529.2012.695254

      https://www.mdpi.com/1420-3049/19/2/2390

      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides?srsltid=AfmBOoo1e2mKmwy-vXjZvAmV-Epz80sS4TfN11C2ShOHlc0FPWkb9_2n

      도움이 되었습니까?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 답변

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      도움이 되었습니까?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 답변

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      도움이 되었습니까?

  4. Hi, Can you provide the information regarding molecular weight of this LPS?

    1 답변

    1. The molecular weight of this product has not been determined. In general, the molecular weight of native LPS may range from 1 – 4 million Daltons or higher. When treated with SDS and heat, the molecular weight of LPS may range from 50 to 100 kDa.

      도움이 되었습니까?

  5. What is the number of acetyl groups attached to the lipid A moiety?

    1 답변

    1. The acyl chain length and number of acyl groups may vary between bacterial species. Certain researchers claim there are 6 acyl groups for LPS from this strain of bacteria. Please refer to those peer reviewed papers below for more information.

      https://www.sciencedirect.com/science/article/pii/S0939641122002338#:~:text=%5B14%5D%2C%20%5B42%5D,in%20a%20single%20LPS%20structure.

      https://pubmed.ncbi.nlm.nih.gov/16934960/

      도움이 되었습니까?

  6. Can you provide information on the solubility and solution stability of this product?

    1 답변

    1. LPS products in solution are expected to be stable in phosphate buffered saline at a concentration of 1 mg/mL when stored at 4 °C for at least one month. For long-term storage, it is recommended to use frozen aliquots. In order to prevent LPS binding to glass, it is advisable to store the solution in silanized containers. If glass containers are used, it is recommended to vortex the solutions for at least 30 minutes to dissolve any bound LPS.

      도움이 되었습니까?

  7. Does the bacterial strain have specific information available, such as whether it originates from a private collection or if there is an associated ATCC number?

    1 답변

    1. Unfortunately, the specific strains used to make L2880 are considered proprietary.

      도움이 되었습니까?

  8. hello, I was wondering what the best way to reconstitute this LPS is

    1 답변

    1. LPS products can be reconstituted in phosphate buffered saline at 1 mg/mL.

      도움이 되었습니까?

  9. Which LPS grade should be used to induce neurotoxicity in rats?

    1 답변

    1. There is no Lipopolysaccharide product offered specifically for the induction of neurotoxicity in rats. Escherichia coli strain O55:B5 has been used to induce oligodendrocyte toxicity to evaluate the neuroprotective effects of certain compounds. In addition, Escherichia coli strain O111:B4 has been cited in the literature as suitable for LPS-induced neurotoxicity. For use with animal models, LPS purified by gel filtration is most commonly used to eliminate the risk of any residual solvents.

      Please see the links below to review the E. coli strains O111:B4 and O55:B5 that have been purified by gel filtration:
      Lipopolysaccharides – Glycobiology
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/research-and-disease-areas/cell-signaling/lipopolysaccharides
      Lipopolysaccharides – selection table
      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

      도움이 되었습니까?

후기

활성 필터

  1. 1 평점-상품평만

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.