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Merck
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MAK263

Sigma-Aldrich

Glucose Colorimetric/Fluorometric Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric or fluorometric tests

응용 분야

cosmetics
food and beverages

검출 방법

colorimetric
fluorometric

관련 질환(들)

endocrinological disorders, diabetes

저장 온도

−20°C

일반 설명

Glucose is a primary energy source that naturally occurs in its free state in fruits and other plant parts. Abnormal glucose levels have been associated with several metabolic dysfunctions such as hypoglycemia, hyperglycemia, and diabetes mellitus. Measurements of glucose levels in tissues and body fluids (such as blood and urine) are often used for the diagnosis of glucose– related disorders. Glucose levels are also monitored to check the efficacy of therapeutics such as insulin and sulphonylureas in type 2 diabetics.

특징 및 장점

Compatible with high-throughput handling systems.

적합성

Suitable for the determination of glucose concentrations in various biological samples, including serum, plasma, food, or growth medium. This kit is also suitable for monitoring glucose levels during fermentation and glucose feeding in protein expression processes.

원리

Glucose is oxidized to generate a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the amount of glucose present. The kit is able to detect 1-10,000 μM of glucose in various samples.

픽토그램

Health hazard
GHS08

신호어

Danger

유해 및 위험 성명서

H334,H412

예방조치 성명서

P261 - P273 - P284 - P501

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
8J20K06060
8H05K06060
5K30K06060
J20K06060
8F22K06060

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리 방문

Ye Du et al.
Frontiers in oncology, 10, 580176-580176 (2021-01-05)
Hypoxia is an important environmental factor and has been correlated with tumor progression, treatment resistance and poor prognosis in many solid tumors, including triple-negative breast cancer (TNBC). Emerging evidence suggests that long noncoding RNA (lncRNA) functions as a critical regulator
Ryan K Perkins et al.
Nutrients, 11(3) (2019-03-03)
The purpose of this investigation was to evaluate the effects of experimental hyperglycemia on oxidative damage (OX), advanced glycation end products (AGEs), and the receptor for AGEs (RAGE) through an in vivo approach. Obese subjects (n = 10; 31.2 ±
Trinidad León-Quinto et al.
Insects, 13(2) (2022-02-26)
The red palm weevil (RPW), Rhynchophorus ferrugineus, is one of the worst palm pests worldwide. In this work, we studied the physiological basis underlying its adaptive strategy against low temperatures. Specifically, we analyzed the main low-molecular-weight biochemical substances acting as
Jake N Barber et al.
The ISME journal, 16(5), 1442-1452 (2022-01-24)
Species loss within a microbial community can increase resource availability and spur adaptive evolution. Environmental shifts that cause species loss or fluctuations in community composition are expected to become more common, so it is important to understand the evolutionary forces
Mike Boger et al.
Frontiers in cell and developmental biology, 10, 918529-918529 (2022-07-26)
The ELMO protein family consists of the homologues ELMO1, ELMO2 and ELMO3. Several studies have shown that the individual ELMO proteins are involved in a variety of cellular and developmental processes. However, it has poorly been understood whether the Elmo
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질문

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  1. Can this kit be used on solid tissue samples? If so, how should the tissue be homogenized?

    1 답변

    1. This kit can be used for tissue samples. To homogenize, please proceed as follows:
      Homogenize the tissue sample in assay buffer using the following protocol:

      Start with approximately 10 mg of tissue (the amount may need to be optimized), wash in cold PBS and resuspend in ~100 uL of assay buffer provided with the kit.
      Homogenize using a Dounce homogenizer or a pestle, on ice to keep the sample cold (10-15 passes).
      Centrifuge at 4°C in a cold microcentrifuge to remove insoluble.
      Collect the supernatant and keep it on ice throughout sample preparation.

      Use a few different volumes of this supernatant (between 2 and 50 uL) and bring them up to a total volume of 50 µl/well with Glucose Assay Buffer in a 96-well plate, as recommended for a typical liquid sample.
      The reason for trying different volumes of samples is to ensure the readings are within the standard curve range.
      Then proceed with the 'Assay Reaction' step.

      Please see the datasheet below for the assay reaction step:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/275/602/mak263bul.pdf

      도움이 되었습니까?

  2. We require a protocol for #MAK263 for my customer

    1 답변

    1. Please see the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/275/602/mak263bul.pdf

      도움이 되었습니까?

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