Isoproterenol is prepared in dH20.
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추천 제품
검출 방법
colorimetric
관련 질환(들)
endocrinological disorders, diabetes
저장 온도
−20°C
일반 설명
Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol.[1] This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase.[2] Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis is associated with obesity, diabetes, and metabolic syndromes.[2]
적합성
Suitable for the measurement of lipolysis in 3T3-L1 and adipocytes.
원리
The Lipolysis (3T3-L1) Colorimetric Assay Kit contains a synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This activates adenylate cyclase, converting ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a colorimetric product with absorbance at 570 nm (A570) proportional to the amount of glycerol present.
키트 구성품 전용
제품 번호
설명
- Lipolysis Assay Buffer
- Lipolysis Wash Buffer
- Glycerol Assay Buffer
- Glycerol Probe, in DMSO
- Glycerol Enzyme Mix
- Glycerol Standard, 100 mM
- Isoproterenol, 10 mM
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
Control of fatty acid and glycerol release in adipose tissue lipolysis.
Langin D.
Comptes rendus biologies, 329(8), 598-607 (2006)
Lipolysis?a highly regulated multi-enzyme complex mediates the catabolism of cellular fat stores.
Lass A, et al.
Progress in Lipid Research, 50(1), 14-27 (2011)
John M Dean et al.
Cell reports, 33(1), 108228-108228 (2020-10-08)
The Mediator complex relays regulatory signals from gene-specific transcription factors to the basal transcriptional machinery. However, the role of individual Mediator subunits in different tissues remains unclear. Here, we demonstrate that MED19 is essential for adipogenesis and maintenance of white
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