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Merck
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MAK086

Sigma-Aldrich

Acetate Colorimetric Assay Kit

sufficient for 100 colorimetric tests

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모든 사진(3)

About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric tests

검출 방법

colorimetric

저장 온도

−20°C

일반 설명

Acetate, the ionized form of acetic acid, is a critical metabolite in fatty acid and carbohydrate metabolism. The majority of acetate is utilized as the conjugate to coenzyme A (CoA), acetyl-CoA. Acetyl-CoA is a precursor to fatty acid and cholesterol synthesis and an important starting compound for the citric acid cycle.[1] Acetyl-CoA also serves as a precursor for the synthesis of acetylcholine and as the donor for acetyl groups for post-translational acetylation reactions of histone and non-histone proteins.[2][2] Acetate is generated from the metabolism of carbohydrates, amino acids, fatty acids, and ethanol.

The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present.

애플리케이션

Acetate colorimetric assay kit has been used to determine acetate concentrations calorimetrically.[3][4]

적합성

Suitable for the detection of acetate in serum, plasma, animal tissues (liver, kidney, muscle, heart etc.), cell culture (adherent or suspension cells) and food.

원리

The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present.

교체됨

제품 번호
설명
가격

Storage Class Code

10 - Combustible liquids

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
8L11K06580
8L08K06580
8L11K06580
8B14K06580
8D15K06580

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Sheng Li et al.
Bioresource technology, 239, 285-293 (2017-05-23)
This study investigated the impact of reverse salt flux (RSF) on microbe community and bio-methane production in a simulated fertilizer driven FO-AnMBR system using KCl, KNO
A refined genome-scale reconstruction of Chlamydomonas metabolism provides a platform for systems-level analyses.
Imam S, et al.
The Plant Journal, 84(6), 1239-1256 (2015)
Acetyl Coenzyme A: A Central Metabolite and Second Messenger
Federico P, et al.
Cell Metabolism, 21(6), 805-821 (2015)
Acetyl-CoA the key factor for survival or death of cholinergic neurons in course of neurodegenerative diseases.
Szutowicz A, et al.
Neurochemical Research, 38(8), 1523-1542 (2013)
Etiological Role of Sulfate-Reducing Bacteria in the Development of Inflammatory Bowel Diseases and Ulcerative Colitis.
Ivan V K, et al.
American Journal of Infectious Diseases and Microbiology , 2(3), 63-73 (2014)
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질문

1–4 / 4 질문  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 답변

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 답변

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. What is the minimum detection limit of this assay? What is the quantification range? 

    1 답변

    1. The kit can detect less than 20 μM of Acetate in various samples. The standard curve ranges from 0 - 10 nmole/well.

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  4. What is the enzymatic reaction and the resulting intermediate substance?

    1 답변

    1. Initially, acetate is converted to an intermediate in the presence of Acetate Enzyme Mix and Acetate Substrate Mix. This intermediate then reduces a colorless probe to a colored product with strong absorbance at 450 nm. The stated intermediate, considered proprietary, is NADH-based."

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