추천 제품
사용
sufficient for 100 colorimetric or fluorometric tests
검출 방법
colorimetric
fluorometric
관련 질환(들)
hematological disorder; cancer
저장 온도
−20°C
일반 설명
Pyruvate kinase (PK) is an enzyme that catalyzes the final step of glycolysis, the transfer of a phosphate group from phospho(enol)pyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase deficiency, due to defects in pyruvate kinase expression or activity, is the second most common cause of hemolytic anemia.
The Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 μmole of pyruvate per minute at 25 °C.
The Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 μmole of pyruvate per minute at 25 °C.
애플리케이션
Pyruvate Kinase Activity Assay Kit has been used to determine the activity of pyruvate kinase in samples.
적합성
Suitable for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc
원리
The Pyruvate Kinase Activity Assay Kit provides a simple and direct procedure for measuring pyruvate kinase activity in a variety of biological samples. Pyruvate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate present. One unit of pyruvate kinase is the amount of enzyme that will transfer a phosphate group from PEP to ADP to generate 1.0 μmole of pyruvate per minute at 25 °C.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
HIF1α modulates cell fate reprogramming through early glycolytic shift and upregulation of PDK1--3 and PKM2.
Prigione A, et al.
Stem Cells, 32(2), 364-376 (2014)
Therapeutic and Biochemical Effects of Garlic (Allium sativum) on Acrylamide Toxicity in Rabbits: Glycolytic Pathway.
Elmahdi B, et al.
Int. J. Pharmacol., 12(4), 429-434 (2016)
Aoi Wakabayashi et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(16), 4434-4439 (2016-04-05)
Whole-exome sequencing has been incredibly successful in identifying causal genetic variants and has revealed a number of novel genes associated with blood and other diseases. One limitation of this approach is that it overlooks mutations in noncoding regulatory elements. Furthermore
Over expression of hyaluronan promotes progression of HCC via CD44-mediated pyruvate kinase M2 nuclear translocation.
L Jing-Huan, et al.
American Journal of Cancer Research, 6(2), 509-509 (2016)
Boa Kim et al.
The Journal of clinical investigation, 128(10), 4543-4556 (2018-09-18)
The M2 isoform of pyruvate kinase (PKM2) is highly expressed in most cancer cells, and has been studied extensively as a driver of oncogenic metabolism. In contrast, the role of PKM2 in nontransformed cells is little studied, and nearly nothing
문서
Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.
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