추천 제품
생물학적 소스
mouse
결합
unconjugated
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
PH-MDMX-55, monoclonal
양식
buffered aqueous solution
분자량
antigen ~80 kDa
종 반응성
human
포장
antibody small pack of 25 μL
농도
~2 mg/mL
기술
indirect ELISA: suitable
western blot: 4-8 μg/mL using total cell extract of HEK-293T cells co-transfected with human MDMX and a specific kinase
동형
IgG2a
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
phosphorylation (pTyr55)
유전자 정보
human ... MDM4(4194)
일반 설명
Monoclonal Anti-phospho-MDMX (pTyr55) (mouse IgG2a isotype) is derived from the hybridoma PH-MDMX-55 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Mouse Double Minute 4 (MDM4) comprises the N-terminal p53 binding domain, a zinc finger, and an acidic region, followed by a really interesting new gene (RING) domain at C-terminus. MDM4 gene is mapped to human chromosome 1q32.1. The RING finger harbors a putative nuclear localization signal.
특이성
Monoclonal Anti-phospho-MDMX (pTyr55) recognizes human phosphorylated MDMX (pTyr55) (approx. 80 kDa).
면역원
synthetic phosphopeptide corresponding to amino acids 50-64 (pTyr55) of human MDMX.
애플리케이션
Monoclonal Anti-phospho-MDMX (pTyr55) antibody produced in mouse may be used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- enzyme-linked immunosorbent assay (ELISA)
생화학적/생리학적 작용
Mouse Double Minute 4 (MDM4 or HDMX or MDMX) undergoes phosphorylation. It is the major inhibitor of p53 transcriptional activity. Despite their significant structural similarity, mouse double minute 2 (MDM2) and MDMX play distinct roles in p53 regulation. The critical and non-redundant role of MDMX and MDM2 in the regulation of p53 in vivo is strikingly demonstrated by the lethality of the mdmx or mdm2 null embryos, which can be fully rescued by the elimination of p53. MDM2 or MDMX are frequently overexpressed in human cancers, leading to the suppression of p53. Studies on MDMX in response to stress conditions, involving post-translational modifications and protein-protein interactions are reported.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
저장 및 안정성
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.
면책조항
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
가장 최신 버전 중 하나를 선택하세요:
Orit Karni-Schmidt et al.
Annual review of pathology, 11, 617-644 (2016-03-30)
For more than 25 years, MDM2 and its homolog MDMX (also known as MDM4) have been shown to exert oncogenic activity. These two proteins are best understood as negative regulators of the p53 tumor suppressor, although they may have additional
Dan Michael et al.
Current opinion in genetics & development, 12(1), 53-59 (2002-01-16)
Cells within an organism are occasionally exposed to either intracellular or environmental stress. Such stress often has genotoxic potential that enhances the probability of cancer. Two gene families, the p53 family (p53, p63 and p73) and the Mdm2 family (Mdm2
Ugo Perricone et al.
MedChemComm, 9(6), 920-936 (2018-08-16)
Molecular dynamics (MD) has become increasingly popular due to the development of hardware and software solutions and the improvement in algorithms, which allowed researchers to scale up calculations in order to speed them up. MD simulations are usually used to
Sue Haupt et al.
Journal of molecular cell biology, 11(3), 231-244 (2019-01-29)
The mouse double minute 4 (MDM4) is emerging from the shadow of its more famous relative MDM2 and is starting to steal the limelight, largely due to its therapeutic possibilities. MDM4 is a vital regulator of the tumor suppressor p53.
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