M5546

Monoclonal Anti-c-Myc antibody produced in mouse

clone 9E10, ascites fluid

• 동의어(들): Anti-c-Myc
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: 9E10, monoclonal
• 포함: 15 mM sodium azide
• 종 반응성: human
• 기술: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 1:100 using c-myc tagged fusion protein
• UniProt 수납 번호: P01106
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MYC(4609)

설명

일반 설명

MYC (MYC proto-oncogene) is a transcriptional factor and oncoprotein. c-myc is a member of MYC gene family. It is located on human chromosome 8q24. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor.
Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.

c-Myc is a transcription factor which is over-expressed in a wide range of cancers.

특이성

Recognizes both N- terminal and C-terminal c-Myc tagged proteins.

면역원

synthetic peptide of the human p62^c-Myc protein.

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.

• Chromatin immunoprecipitation (1 paper)
• Enzyme-linked immunosorbent assay (1 paper)
• Immunoprecipitation (5 papers)
• Western Blotting (5 papers)
• Western blotting following immunoprecipitation(1 paper)

Monoclonal anti-c-Myc antibody produced in mouse has been used for Western blotting and immunohistochemistry.
Monoclonal Anti-c-Myc antibody has also been used in:

• the detection of horseradish peroxidase conjugate in phosphate buffered saline tween-20 (PBST) and 65-kDa mannoprotein (MP65) Dabs
• immunoblotting
• immunoprecipitation
• biochemical and histological studies

생화학적/생리학적 작용

The cellular myelocytomatosis (c-myc) oncogene plays a major role in cellular proliferation, differentiation and apoptosis. It acts as a transcriptional regulator of gene expression. MYC (MYC proto-oncogene) may be essential for cancer initiation, promotion and therapy resistance. Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in the poor outcome and invasive treatment, when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP).

c-Myc is a regulator of the cell cycle and aids in the growth of tumors in the liver. Translocations in the gene encoding it have been shown to be linked to Burkitt lymphoma. It has been studied that c-Myc targets many protein-coding genes which enhances the oncogenic properties of c-Myc.

물리적 형태

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

저장 및 안정성

For continuous use, store at 2-8 °C for a maximum of one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable