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Merck
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L8754

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder

동의어(들):

Phaseolus vulgaris agglutinin, PHA

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크기 선택

5 MG
₩67,802
25 MG
₩179,046
50 MG
₩308,560

₩67,802

출고 가능일2025년 4월 07일세부사항

모든 사진(2)

크기 선택

보기 변경
5 MG
₩67,802
25 MG
₩179,046
50 MG
₩308,560

About This Item

EC Number:
232-718-7
UNSPSC 코드:
12352202
NACRES:
NA.32

₩67,802

출고 가능일2025년 4월 07일세부사항

생물학적 소스

Phaseolus vulgaris

양식

lyophilized powder

효능

≤62.5 μg per mL agglutination activity
≤10 μg/mL mitogenic activity

불순물

salt, free

저장 온도

2-8°C

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관련 카테고리

일반 설명

PHAs are carbohydrate-binding proteins. There are several types of PHAs identified, predominantly in leguminous plants.[1]
Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants (esp. in legumes). It is obtained from Phaseolus vulgaris (red kidney beans) and acts as a mitogen in human leucocyte culture.[2]

애플리케이션

It has also been used as an antigen to measure in vivo T-cell-mediated immune responsiveness.[3]
Lectin from Phaseolus vulgaris (red kidney bean) has been used in phytohemagglutinin (PHA) assay[4] and during assessment of cellular and humoral immunity.[5]

생화학적/생리학적 작용

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

분석 메모

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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시험 성적서(COA)

Lot/Batch Number
0000435719
0000435719
0000427537
0000427537
0000414307

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문서 라이브러리 방문

Laura Gangoso et al.
Oecologia, 178(4), 1113-1123 (2015-04-04)
Genetic color polymorphism is widespread in nature. There is an increasing interest in understanding the adaptive value of heritable color variation and trade-off resolution by differently colored individuals. Melanin-based pigmentation is often associated with variation in many different life history
A R S Badari Nath et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 134, 143-147 (2014-07-10)
Phytohaemagglutinin (PHA) is a lectin obtained from Phaseolus vulgaris (red kidney beans), that acts as a mitogen in human leucocyte culture and is commercially available from Gibco. This PHA (Gibco) was found to be very expensive, hence other inexpensive sources
Phytohemagglutinin content in fresh kidney bean in China
Sun Y, et al.
International journal of food properties, 22(1), 405-413 (2019)
Andrew Z Wang et al.
The Journal of experimental biology, 223(Pt 20) (2020-09-13)
Limited resources must be partitioned among traits that enhance fitness. Although survival-related traits often trade off with reproduction, survival-related traits themselves may trade off with each other under energy limitations. Whole-organism performance and the immune system both enhance survival, yet
Qianting Yang et al.
Thorax, 75(7), 576-583 (2020-03-24)
Biomarker-based tests for diagnosing TB currently rely on detecting Mycobacterium tuberculosis (Mtb) antigen-specific cellular responses. While this approach can detect Mtb infection, it is not efficient in diagnosing TB, especially for patients who lack aetiological evidence of the disease. We
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문서

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

질문

  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 답변

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

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