L2895

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Isolectin B₄ (BSI-B₄), FITC conjugate, lyophilized powder

• 동의어(들): Bandeirea simplicifolia agglutinin, BS-I
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 결합: FITC conjugate
• Quality Level: 200
• 양식: lyophilized powder
• 효능: <50 μg per mL agglutination activity (using human blood group B erythrocytes)
• 구성: Protein, ≥70% Lowry (balance sodium citrate)
• 라벨링 범위: ≥1 mol FITC per mol lectin
• 저장 온도: 2-8°C

설명

일반 설명

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.[1]

애플리케이션

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used for microglia detection[2] in murine astrocytes culture by staining the culture with lectin BS1-B4.[3] It has also been used in the preparation of staining solution to incubate the tissue sections.[4]

생화학적/생리학적 작용

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling, because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.[1]

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

포장

Package size based on protein content by Lowry.

기타 정보

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B₄, BSI-AB₃, BSI-A₂B₂, BSI-A₃B and BSI-A₄. BSI-B₄ is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A₄ has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

분석 메모

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)