KEM0031

Klenow Fragment

Ultra-pure enzyme for nucleic acid modifications

속성

• Grade: for molecular biology
• 분석: >99% (SDS-PAGE)
• 양식: buffered aqueous solution
• 특이 활성도: 5,000 U/mg
• 농도: 5,000 U/mL
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Klenow Fragment is a mesophilic DNA polymerase derived from the E.coli Polymerase I DNA-dependent repair enzyme. The enzyme exhibits DNA synthesis and proofreading (3′ → 5′) nuclease activities, and, in the absence of the holoenzyme′s (5′→3′) nuclease domain, displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene.

애플리케이션

Suitable for:

• DNA blunting by fill-in of 5′ overhang
• Second strand cDNA synthesis
• Sequencing
• Site-specific mutagenesis

특징 및 장점

• Ultra-purification process for ultimate enzyme performance
• Highest quality specifications for ultimate product consistency
• Undetectable DNA and nuclease contamination

성분

Supplied with:KEM0042B (10X Blue Buffer)

단위 정의

1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37° C.

물리적 형태

Supplied in 100 mM KPO₄, 1.0 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 7.4 @ 25° C.

기타 정보

Source of protein: A recombinant E. coli strain carrying the Klenow Fragment gene.

Unit size: 2,500 U

안전성 정보

• Storage Class Code: 10 - Combustible liquids