I4753

Invertase from Candida utilis

Grade X, ≥300 units/mg solid

• 동의어(들): β-D-Fructofuranoside fructohydrolase, β-Fructofuranosidase, Saccharase
• CAS Number: 9001-57-4
• 효소 위원회 번호: 3.2.1.26 (BRENDA, IUBMB)
• EC Number: 232-615-7
• MDL number: MFCD00131410
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 유형: Grade X
• 형태: solid
• 특이 활성도: ≥300 units/mg solid
• 분자량: ~260 kDa
• 외래 활성: α-galactosidase (melibiase), essentially free
• 저장 온도: −20°C

설명

일반 설명

Invertase from Candida utilis is secreted in the periplasm. The molecular weight of invertase from C. utilis is 300 kDa. It is a dimer of identical subunits. It is a N-glycosylated protein, rich in mannose oligosaccharide in its structure. The invertase encoding gene of C. utilis shares high homology with SUC2 gene of Saccharomyces cerevisiae.

애플리케이션

Invertase from Candida utilis has been used:

• as a supplement in YE liquid medium to observe for morphological changes in Streptomyces sp.
• to determine the sucrose content in ethanolic extract of leaf tissues
• to determine the total non-structural carbohydrates (TNC) in root tissues

This enzyme is useful for enzymatic determination of saccharose and for the structure investigation of carbohydrates containing β-D-fructofuranoside residue.

생화학적/생리학적 작용

Invertase acts on sucrose and cleaves it into glucose and fructose. It is used in the preparation of invert syrup with equimolar concentration of fructose and sucrose. It finds its applications in confectionaries and in the production of alcoholic beverages, glycerol and lactic acid. Invertase is also used in the preparation of artificial honey, plasticizers for cosmetic industries.

물리적 특성

Michaelis constant : 1.5x10 ^-2M (Saccharose)
Structure : Glycoprotein containing ca. 50% of carbohydrates
Optimum pH : 3.5 - 4.0 Optimum temperature : 60 - 70o^C
pH Stability : pH 4.0 - 6.0 (50o^C, 10min)
Thermal stability : below 60o^C (pH 4.5, 10min)
Substrate specificity : The enzyme hydrolyzes saccharose and raffinose, but does not
hydrolyze inulin and melezitose.6⁾

단위 정의

One unit will hydrolyze 1.0 μmole of sucrose to invert sugar per min at pH 4.5 at 55°C.

제조 메모

Chromatographically purified

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)