I0634

Ionomycin calcium salt

from Streptomyces conglobatus, ≥98% (HPLC), powder, Ca2+ ionophore

• 동의어(들): Ionomycin (calcium), SQ23377 calcium
• 실험식(Hill 표기법): C₄₁H₇₀CaO₉
• CAS Number: 56092-82-1
• Molecular Weight: 747.07
• Beilstein: 1416163
• MDL number: MFCD00083634
• UNSPSC 코드: 12352200
• PubChem Substance ID: 329815321
• NACRES: NA.77

속성

• 제품명: Ionomycin calcium salt from Streptomyces conglobatus, powder, ≥98% (HPLC)
• Quality Level: 300
• 분석: ≥98% (HPLC)
• 양식: powder
• 저장 조건: desiccated; protect from light
• 색상: white to off-white
• solubility: DMSO: 10 mg/mL; ethanol: soluble
• 항생제 활성 스펙트럼: Gram-positive bacteria
• 동작 모드: cell membrane | interferes
• 저장 온도: 2-8°C
• SMILES string: C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)C\C=C\[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O
• InChI: 1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1
• InChI key: WKRWUYKLUMMAKG-WYGBAUISSA-L

설명

일반 설명

Calcium Ionomycin is a polyether antibiotic made by Streptomyces conglobatus. It can remove Ca²⁺ and divalent cations from an aqueous into an organic phase. Ionomycin enhances the influx of Ca²⁺ by triggering store-regulated cation entry rather than acting directly on the plasma membrane.[1] It can induce apoptosis, potentially by activating a hidden enzyme that responds to calcium. Since calcium salt of ionomycin serves as a highly efficient transporter of Ca²⁺, it offers substantial benefits for studying the transport of Ca²⁺ across biological membranes. Ionomycin is employed to balance intracellular and extracellular calcium ion concentrations for in situ calibrations of fluorescent indicators.

Additionally, it is a narrow spectrum antibiotic effective against Gram-positive bacteria, obtained in pure form from the fermentation broths of Streptomyces conglobatus. It is unique amongst known polyether antibiotics due to its exceptional UV absorption maximum at 300 nm and its high affinity for calcium ions.[2]

애플리케이션

Ionomycin calcium salt from Streptomyces conglobatus has been used as a media supplement to culture:

• peripheral blood mononuclear cells (PBMCs) for immunologic assessment[3]
• oocytes for parthenogenetic activation[4]
• T cell subsets for the detection of intracellular cytokines, carboxyfluorescein diacetate succinimidyl ester (CFSE) proliferation assay, and antigen-specific suppressive function assay[5]
• in combination with phorbol12-myristate 13-acetate (PMA) to stimulate T-cell activation in various cells[6]
[7]
• as sperm capacitation medium supplement to study its impact on in vitro fertilization and increasing the efficiency of bovine embryo development[8]
• as a positive control for the detection of intracellular Ca²⁺ in adipose-derived stem cells (ASCs)(4)[5]

생화학적/생리학적 작용

Ca²⁺ ionophore that is more effective than A23187 as a mobile ion carrier for Ca²⁺.

Ca²⁺ ionophore that is more effective than A23187 as a mobile ion carrier for Ca²⁺; non-fluorescent; used to study Ca²⁺ transport across biological membranes; induces apoptotic degeneration of embryonic cortical neurons.

Ionomycin is an ionophore, widely used for validation and calibration of calcium analysis.[9]

제조 메모

Store ionomycin in powder form desiccated and protected from light at 2-8 °C. Under these conditions the product is stable for 3 years. Store stock solutions in ethanol or DMSO at -20 °C, protected from light. Under these conditions the stock solutions are stable for several months. For short term use (up to 6 weeks) the stock solutions maybe stored at 2-8 °C protected from light.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302
• 예방조치 성명서: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves