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Merck
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문서

H3631

Sigma-Aldrich

Hyaluronidase from bovine testes

Type VI-S, lyophilized powder, 3,000-15,000 units/mg solid

동의어(들):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

CAS Number:
37326-33-3
효소 위원회 번호:
3.2.1.35 (BRENDA, IUBMB)
EC Number:
253-464-3
MDL number:
MFCD00131351
UNSPSC 코드:
12352204
NACRES:
NA.54

생물학적 소스

bovine testis

결합

conjugate (Glucosaminoglycan)

유형

Type VI-S

형태

lyophilized powder

특이 활성도

3,000-15,000 units/mg solid

분자량

~55 kDa (four subunits of 14 kDa each)

UniProt 수납 번호

Q5E985
Q8SQG8

관련 질환(들)

cancer

저장 온도

−20°C

유전자 정보

cow ... HYAL1(515397) , HYAL2(281838)

유사한 제품을 찾으십니까? 방문 제품 비교 안내

생화학적/생리학적 작용

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

단위 정의

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

제조 메모

Chromatographically purified

기타 정보

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

픽토그램

Health hazard
GHS08

신호어

Danger

유해 및 위험 성명서

H334

예방조치 성명서

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Dong-Ru Ho et al.
International journal of urology : official journal of the Japanese Urological Association, 18(7), 525-531 (2011-05-25)
To investigate how hyaluronic acid (HA) affects nerve growth factor (NGF) production and bladder overactivity in a cyclophosphamide (CYP)-induced cystitis rat model. Female Sprague-Dawley rats received three intermittent intraperitoneal injections of CYP (75 mg/kg) or saline. Before or after CYP injection
Tongcui Ma et al.
eLife, 11 (2022-07-06)
High-parameter single-cell phenotyping has enabled in-depth classification and interrogation of immune cells, but to date has not allowed for glycan characterization. Here, we develop CyTOF-Lec as an approach to simultaneously characterize many protein and glycan features of human immune cells
Tongcui Ma et al.
eLife, 9 (2020-05-27)
The female reproductive tract (FRT) is the most common site of infection during HIV transmission to women, but viral remodeling complicates characterization of cells targeted for infection. Here, we report extensive phenotypic analyses of HIV-infected endometrial cells by CyTOF, and
Helen M Mcgettrick et al.
Methods in molecular biology (Clifton, N.J.), 1591, 121-142 (2017-03-30)
Methods are described for analyzing adhesion and migration of isolated lymphocytes on endothelial cell monolayers which have been cocultured with different mesenchymal stromal cells, with or without additional cytokine treatment. The different cells types are grown on opposite sides of
Sialic acids rather than glycosaminoglycans affect normal and sickle red blood cell rheology by binding to four major sites on fibrinogen.
Frank Gondelaud et al.
American journal of hematology, 95(4), E77-E80 (2020-01-07)
모든 관련된 서류 보기

문서

What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

프로토콜

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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