This product is sufficient for ≤10 assays. When reconstituted with 10 ml deionized water, each vial will yield a solution with a pH of approximately 7.3, containing the following components: 0.3 mM 4-Hydroxyazobenzene-2-carboxylic acid (HABA), 0.45 mg/ml avidin, 0.3 M sodium chloride (NaCl), 0.01 M N-[2-Hydroxyethyl]piperazine-NN-[2-ethanesulfonic acid] (HEPES), a buffer with a pKa of 7.5, 0.01 M magnesium chloride (MgCl2), and 0.02% sodium azide as a preservative.
추천 제품
양식
lyophilized powder
Quality Level
사용
sufficient for ≤10 assays
색상
orange
적합성
suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples
저장 온도
2-8°C
일반 설명
4-Hydroxyazobenzene-2-carboxylic acid (HABA) is an avidin-binding dye that can be stoichiometrically displaced by biotin. The subsequent change in absorption at A500 provides a basis for spectrophotometric determination of biotin levels in biological samples.
HABA/Avidin Reagent is specific for chromogenic applications that depend upon quantification of biotin.
애플리케이션
HABA/Avidin Reagent is suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples.
Reagent is capable of detecting biotin at μMolar concentrations (i.e., 1 nmole per ml). Typically used to quantitate biotin in biotinylated compounds.
재구성
Prepares a ready-to-use HABA/avidin solution in buffered saline, pH approx. 7.3, with preservative when reconstituted with 10 ml deionized water.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
관련 제품
제품 번호
설명
가격
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
A Einspanier et al.
Biology of reproduction, 61(2), 512-520 (1999-07-20)
Relaxin is a peptide hormone with a broad range of biological activities, related not only to parturition and lactation but possibly also to decidualization, implantation, and early pregnancy. The present study was designed to investigate the secretion pattern of relaxin
R S Niedbala et al.
Journal of biochemical and biophysical methods, 13(4-5), 205-210 (1986-11-01)
Parameters and conditions of an enzyme based assay for biotin and avidin are presented. Biotinylated glucose-6-phosphate dehydrogenase when complexed with avidin becomes inactivated. Thus it was possible to construct a competitive assay system for biotin. The assay is sensitive between
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I would like to know how many assays can be done with a VIAL KIT and how much material it contains,
1 답변-
도움이 되었습니까?
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how much HABA is in the vial
1 답변-
When reconstituted to 10mL, the vial will contain 0.3 mM HABA. Based on the molecular weight of HABA, 242.23 gmol, the calculated mass per vial is 726.69 ug. Please see the link below to review additional informaition available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/378/451/h2153dat.pdf도움이 되었습니까?
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