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Merck
모든 사진(2)

주요 문서

H1666

Sigma-Aldrich

Anti-HOXC4 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

동의어(들):

Anti-Homeobox 3E, Anti-Homeobox C4, Anti-Hox-3E CP19

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rabbit

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

양식

buffered aqueous solution

분자량

antigen ~40 kDa

종 반응성

human

농도

~1.0 mg/mL

기술

immunoprecipitation (IP): 2.5-5 μg/mL using lysates of HEK-293T cells over-expressing human HOXC4
indirect immunofluorescence: 1-2 μg/mL using paraformaldehyde-fixed HEK-293T cells over-expressing human HOXC4
western blot: 0.5-1 μg/mL using lysates of HEK-293T cells over-expressing human HOXC4

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... HOXC4(3221)
mouse ... Hoxc4(15423)
rat ... Hoxc4(24459)

일반 설명

The Homeobox C4 (HOXC4) is located on the human chromosome at 12q13.13.

특이성

Anti-HOXC4 (N-terminal) recognizes human HOXC4 (also known as Hox-3E and CP19).

애플리케이션

Anti-HOXC4 (N-terminal) antibody produced in rabbit may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

생화학적/생리학적 작용

Homeobox C4 (HOXC4) transcription factor/protein stimulates the action of activation-induced cytosine deaminase (AID). The HOXC4 gene is upregulated due to the estrogen-estrogen receptor complexes. HOXC4 gene is expressed in the proliferating lymphocytes of B-, T- and natural killer (NK) cell lineage. This gene expression is elevated with the progression of lymphoid differentiation.

물리적 형태

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

J van Oostveen et al.
Leukemia, 13(11), 1675-1690 (1999-11-11)
In the last decade it has become clear that homeobox containing genes (HOX genes) not only play a significant role in regulating body formation, but in addition, they are contributing to organization and regulation of hematopoiesis. Modern molecular technologies showed
R Meazza et al.
Blood, 85(8), 2084-2090 (1995-04-15)
We have analyzed the expression of homeoproteins of the HOX family in resting and activated lymphoid cells and in neoplastic lymphoid cell lines by the use of monoclonal antibodies (MoAbs) already shown to react with the homeoproteins HOXA10, HOXC6, and
Seok-Rae Park et al.
Cytokine, 61(2), 608-613 (2012-11-28)
Activation-induced cytidine deaminase (AID) plays a key role in B cell immunoglobulin (Ig) class switch recombination (CSR) and somatic hypermutation (SHM). We have previously reported that the highly conserved homeodomain HoxC4 transcription factor binds to the Aicda (AID gene) promoter
Frank Mendrzyk et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 12(7 Pt 1), 2070-2079 (2006-04-13)
Pathogenesis of ependymomas is still poorly understood and molecular markers for risk-adapted patient stratification are not available. Our aim was to screen for novel genomic imbalances and prognostic markers in ependymal tumors. We analyzed 68 sporadic tumors by matrix-based comparative
Thach Mai et al.
The Journal of biological chemistry, 285(48), 37797-37810 (2010-09-22)
Estrogen enhances antibody and autoantibody responses through yet to be defined mechanisms. It has been suggested that estrogen up-regulates the expression of activation-induced cytosine deaminase (AID), which is critical for antibody class switch DNA recombination (CSR) and somatic hypermutation (SHM)

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