H0163

Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal

clone HDAC4-144, purified from hybridoma cell culture

• MDL number: MFCD05665442
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: HDAC4-144, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~140 kDa
• 종 반응성: human, rat, mouse
• 향상된 검증: independent; Learn more about Antibody Enhanced Validation
• 농도: ~2 mg/mL
• 기술: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 1-2 μg/mL using total cell extracts of NIH3T3 fibroblasts cells
• 동형: IgG2a
• UniProt 수납 번호: P56524
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HDAC4(9759) ; mouse ... Hdac4(208727) ; rat ... Hdac4(363287)

설명

일반 설명

Monoclonal Anti-Histone Deacetylase 4 (HDAC4) (mouse IgG2a isotype) is derived from the HDAC4-144 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human HDAC4 with C-terminal added lysine, conjugated to KLH. HDAC4 belongs to the class II of Mammalian HDACs.

애플리케이션

Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting[1]
• immunocytochemistry
• immunoprecipitation

생화학적/생리학적 작용

Histone deacetylase (HDAC) catalyzes the deacetylation of histones. HDAC4 is implicated in transcriptional repression. It dynamically migrates between nucleus and cytoplasm through its nuclear import and export signals. Interaction of HDAC4 with 14-3-3 and myocyte enhancer factor-2 (Mef2) proteins disturbs such shuttling and thus directs HDAC4 to the cytoplasm and the nucleus, respectively.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable