추천 제품
형태
solid
특징
wetted part: no
포장
pack of 25 g
제조업체/상표
Cytiva RPN6302
색상
Yellowish
solubility
cold water: soluble (Easily soluble in cold and hot water)
저장 온도
2-8°C
일반 설명
Used for the efficient alkylation of thiols while minimizing oxidation of competing thiol pairs in protein samples.
애플리케이션
Iodoacetamide has been used:
- as a component in urea-sodium dodecyl sulfate (SDS) buffer for yeast cell lysis in immunoprecipitation assay
- in equilibration of pH gradient strips during the second dimension run in isoelectric focusing
- as a protein alkylation agent
생화학적/생리학적 작용
Iodoacetamide (IAA) modifies thiol groups in proteins by S-carboxyamidomethylation. Iodoacetamide is an alkylating agent. IAA is commonly used to analyze the key role of cysteine residues in the catalytic reactions of enzymes. It is also used in the derivatization of cysteine residues in proteins for proteomic analysis. IAA irreversibly inhibits glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
특징 및 장점
- Ultrapure, proteomic-grade iodoacetamide is used for efficient alkylation of thiols while minimizing oxidation of the competing thiol pairs in protein samples.
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
신호어
Danger
유해 및 위험 성명서
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Differential effects of iodoacetamide and iodoacetate on glycolysis and glutathione metabolism of cultured astrocytes
Schmidt M and Dringen R
Frontiers in Neuroenergetics, 1(13), 1-1 (2009)
COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal
Xu P, et al.
eLife, 6, e28342-e28342 (2017)
Differential Protein Expression in the Nucleus Accumbens and Amygdala of Lewis and Fischer 344 Rats, and its Relevance in Drug Addiction
Roura-Martinez D, et al.
Journal of drug and alcohol research, 6(3), b1-b8 (2014)
Proteomic analysis of palmitoylated platelet proteins
Dowal L, et al.
Blood, 118(13), e62-e73 (2011)
프로토콜
This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.
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