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문서

GE29-0485-81

HiTrap® Protein G HP

Cytiva 29-0485-81, pack of 1 mL

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About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

포장

pack of 1 mL

제조업체/상표

Cytiva 29-0485-81

재고 정보

not available in North America

저장 조건

(20% Ehtanol)
(20% Ethanol)

파라미터

flow rate
42 psi

베드 크기

7 mm × 25 mm

베드 용적

1 mL

컬럼 I.D.

7 mm

기질

highly cross-linked 6% agarose

평균 부품 크기

34 μm

cleaning

2-9

working range

3-9

용량

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
25 mg binding capacity(human IgG/column)

저장 온도

2-8°C

일반 설명

HiTrap Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

특징 및 장점

High binding capacity of 25 mg IgG/ml medium and fast binding kinetics.
34 μm bead size for high performance, high-resolution purifications.
Binds broad range of different species and subclasses of IgG.
Simple and proven method giving reproducible results.
Designed for use with syringe, peristalic pump, or chromatography system.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

HiTrap is a registered trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Philipp A Kronenberg et al.
Frontiers in cellular and infection microbiology, 13, 1162530-1162530 (2023-04-04)
Alveolar (AE) and cystic echinococcosis (CE) are severe parasitic zoonoses caused by the larval stages of Echinococcus multilocularis and E. granulosus sensu lato, respectively. A panel of 7 monoclonal antibodies (mAbs) was selected against major diagnostic epitopes of both species.

문서

Designing purification methods for antibodies ensures quality and quantity adequacy for research and industrial purposes.

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

모두 보기

프로토콜

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

General instructions for affinity purification using HiTrap columns from Cytiva.

This page shows how to prepare samples for purification with affinity chromatography.

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