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문서

GE28-9909-45

Sigma-Aldrich

Superdex® 200 Increase 5/150 GL

Cytiva 28-9909-45, column L × I.D. 15 cm × 5 mm, 8.6 μm particle size

동의어(들):

Size-exclusion chromatography column

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About This Item

UNSPSC 코드:
41115700
NACRES:
SB.52

material

glass column
polyethylene filter

제품 라인

Superdex®

분자량

10000-600000 Da (globular proteins)

제조업체/상표

Cytiva 28-9909-45

포장

1 ea of

파라미터

<0.75 mL/min flow rate
<0.75 ml/min flow rate
<3.0 Mpa (725 psi)
4-50 μL sample volume

베드 용적

~3 mL

컬럼 길이 × I.D.

15 cm × 5 mm

기질

agarose dextran composite
polymeric

기질 활성군

phenyl phase

입자 크기

8.6 μm

cleaning

1-14

working range

3-12

분리 기술

size exclusion (SEC)

관련 카테고리

일반 설명

Superdex 200 is a column for rapid purity check and screening by high resolution gel filtration for proteins with molecular weights between 10 000 and 600 000, such as antibodies. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

적합성

Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride
Well suited for rapid purity checks and quick screenings of experimental conditions.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

Superdex is a registered trademark of Cytiva

픽토그램

Flame
GHS02

신호어

Warning

유해 및 위험 성명서

H226

Storage Class Code

3 - Flammable liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

프로토콜

Performing a Separation with Superdex

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Refolding: Ion Exchange Chromatography

Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.

Sample Preparation for Size Exclusion Chromatography

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

Solubilization of Membrane Proteins

This page shows how to solubilize membrane proteins with products from Cytiva.

관련 콘텐츠

Separation Options with Superdex®

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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