추천 제품
포장
pkg of 1 ea
제조업체/상표
Cytiva 28-9365-51
파라미터
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
베드 크기
16 mm × 100 mm
베드 용적
20 mL
컬럼 I.D.
16 mm
Matrix
6% cross-linked agarose
입자 크기
45-165 μm
평균 직경
90 μm
cleaning
2-14(Ni2+-stripped medium.)
작동 범위
3-12(Ni2+-stripped medium.)
용량
~40 mg binding capacity(histidine-tagged protein)
적합성
suitable for bioprocess medium
관련 카테고리
일반 설명
HisPrep™ FF 16/10 columns are prepacked with Ni Sepharose™ 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose™ 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
특징 및 장점
- Optimized for convenient scale-up purification of histidine-tagged proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
- Negligible leakage of Ni2+.
- High binding capacity, approx. 40 mg/mL medium.
- Convenient and time-saving 20 mL prepacked HiPrep™ format.
저장 및 안정성
분석 메모
기타 정보
법적 정보
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
가장 최신 버전 중 하나를 선택하세요:
문서
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
프로토콜
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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