GE28-9042-65

illustra^™ Blood Mini , 250

Cytiva 28-9042-65

• 동의어(들): Blood Mini Kit, illustra Blood Kit
• UNSPSC 코드: 41105501
• NACRES: NA.55

속성

• 포장: pkg of 250 ea
• 제조업체/상표: Cytiva 28-9042-65
• 용량: 60 μg binding capacity

설명

일반 설명

Blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells.

Genomic DNA is found in the chromosomes and it is much longer and higher in molecular weight than plasmid DNA. Genomic DNA is the code for the genome, and this is what is needed to do genome sequencing.

For sequencing it is desirable that the genomic DNA extraction technique used allows the collection of intact genomic DNA in the longest fragments possible. A kit format can help standardize the procedures used to prepare tissue and cell genomic DNA samples for analysis.

The purification process involves minimal shearing resulting in the production of 5 to 10 μg of good quality, intact genomic DNA from a 200 μL sample. Input sample volumes can vary from 50 μL to 1 mL.

The method uses a simple genomic DNA purification protocol. This protcol that uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a column-based, novel silica membrane. This results in high molecular weight DNA that can be used downstream (e.g., in bone marrow genomic DNA for next generation sequencing).

The elution volume can be modified as necessary to obtain the appropriate genomic DNA concentration for most downstream molecular biological applications.

The genomic DNA kit contains spin columns prepacked with a novel silica membrane, lyophilized proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.

애플리케이션

Suitable for use in molecular biology applications including standard, multiplex and quantitative PCR; cloning; haplotyping; restriction enzyme digestion; and genotyping.

특징 및 장점

• High-quality genomic DNA extraction from blood with a greater proportion of higher molecular weight DNA and less shearing
• Demonstrated application to various types of whole blood including human, horse, rabbit, rat, and mouse
• Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and color-coded kit components
• Verified in several downstream applications including reverse transcription polymerase chain reaction (RT-PCR), endpoint PCR, multiplex PCR, and restriction enzyme digests

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

illustra is a trademark of Cytiva

안전성 정보

• 픽토그램: GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H302,H318,H315,H412
• 예방조치 성명서: P280 - P273 - P301 + P312 - P305 - P310 - P501
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3