추천 제품
생물학적 소스
Escherichia coli (NM 989)
Quality Level
재조합
expressed in E. coli
설명
recombinant form of the enzyme from T4 phage
형태
solution
특이 활성도
~3000 units/mg protein
포장
pkg of 100 U (10481220001 [1 U/μl])
pkg of 500 U (10716359001 [1 U/μl])
pkg of 500 U (10799009001 [5 U/μl])
제조업체/상표
Roche
파라미터
4-22 °C optimum reaction temp.
기술
activity assay: suitable
최적 pH
7.2-7.8
저장 온도
−20°C
관련 카테고리
일반 설명
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.
Contents
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.
Contents
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.
특이성
Heat inactivation: The enzyme can be inactivated by heating (65 °C, 10 minutes).
애플리케이션
Ligation of sticky- and blunt-ended DNA fragments.
품질
Quality
Tested for the absence of deoxyribonucleases and exonucleases, according to the current Quality Control procedures.
Tested for the absence of deoxyribonucleases and exonucleases, according to the current Quality Control procedures.
단위 정의
One unit of T4 DNA ligase is the amount of enzyme activity that converts 1 nmol 32P from pyrophosphate into Norit-absorbable material in 20 minutes at +37 °C. One unit corresponds to 0.2 U determined in the exonuclease III resistance assay. Dilution buffer: 50 mM Tris-HCl, 10 mM dithioerythritol, bovine serum albumin, 500 μg/ml, pH 7.6 (+25 °C).
Volume Activity: 1 x 103 U/ml; 5 x 103 U/ml
Volume Activity: 1 x 103 U/ml; 5 x 103 U/ml
제조 메모
Activator: Mg2+ (10mM), DTT
Working solution: For dilution of the enzyme Roche recommends using a buffer containing the components of the storage buffer: 20 mM Tris-HCl, 60 mM KCl, 1 mM EDTA, 5 mM dithoerythritol, 50% glycerol (v/v), pH 7.5 (4 °C).
Working solution: For dilution of the enzyme Roche recommends using a buffer containing the components of the storage buffer: 20 mM Tris-HCl, 60 mM KCl, 1 mM EDTA, 5 mM dithoerythritol, 50% glycerol (v/v), pH 7.5 (4 °C).
기타 정보
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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