콘텐츠로 건너뛰기
Merck
모든 사진(1)

주요 문서

GE28-9031-34

Protein G High Performance Spintrap™

Cytiva 28-9031-34, pack of 16 columns

동의어(들):

Protein G Column

로그인조직 및 계약 가격 보기
모든 사진(1)

About This Item

UNSPSC 코드:
41106500
NACRES:
NA.25
가격 및 재고 정보를 현재 이용할 수 없음 고객지원팀으로 연락바랍니다.

ligand

recombinant protein G lacking albumin-binding region

특징

wetted part: no

포장

pack of 16 columns

제조업체/상표

Cytiva 28-9031-34

저장 조건

(20% Ehtanol)
 (20% Ethanol)

Matrix

highly cross-linked 6% agarose

평균 부품 크기

34 μm

cleaning

2-9

작동 범위

3-9

용량

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
 >1 mg binding capacity(human IgG/column)

저장 온도

2-8°C

관련 카테고리

일반 설명

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
protein G HP SpinTrap™ is a prepacked, single-use spin column containing protein G Sepharose™ High Performance.

애플리케이션

Protein G Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).


There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

특징 및 장점

  • For simple, small-scale purification of antibodies or enrichment of specific Proteins in prepacked spin columns that are ready to use.
  • Classic and cross-link protocols provide flexibility.
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provides high yield.
  • Prepacked with Protein G Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
  • Easy scale-up with HiTrap® Protein G HP prepacked columns.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

픽토그램

Flame
GHS02

신호어

Warning

유해 및 위험 성명서

H226

Storage Class Code

3 - Flammable liquids

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

도움이 필요하시면 연락하세요. 고객 지원 부서

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

프로토콜

Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.