추천 제품
material
resin (Sephadex G-25 DNA grade F)
무균
non-sterile
제조업체/상표
Cytiva 27-5325-01
포장
pkg of 50 columns
작업 용적
10 —100 μL
입자 크기
20-80 μm (dry)
공극 크기
5000 Da MWCO
작동 범위
2-13
관련 카테고리
일반 설명
MicroSpin™ G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.
특징 및 장점
- For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin-column chromatography.
- Excellent for rapid purification of newly synthesized oligonucleotides >10-mers in 100 to 150 μl of deprotection solution using spin-column chromatography.
- Convenient: Prepacked with Sephadex® G-25 DNA Grade and pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide.
- Ready to use: Requires less than 4 min from sample application to collection of purified product.
- Tested in nickase, single and double-stranded exonuclease and RNase assays.
- Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 μl.
저장 및 안정성
분석 메모
법적 정보
Storage Class Code
12 - Non Combustible Liquids
가장 최신 버전 중 하나를 선택하세요:
문서
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
프로토콜
DNA cleanup using illustra™ GFX kits from Cytiva.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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