GE17-5268-02

Ni Sepharose^™ High Performance

Cytiva 17-5268-02, pack of 100 mL

• 동의어(들): High Performance Ni Resin, Ni Sepharose
• UNSPSC 코드: 41106500
• NACRES: NA.56

속성

• 포장: pack of 100 mL
• 제조업체/상표: Cytiva 17-5268-02
• 기질: highly cross-linked 6% agarose
• 평균 부품 크기: 34 μm
• cleaning in place: 2-14(Ni²⁺-stripped medium.)
• working range: 3-12(Ni²⁺-stripped medium.)
• 용량: ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

설명

일반 설명

Ni Sepharose^™ High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose^™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni²⁺, which will selectively retain proteins with exposed histidine groups.

The high stability and broad compatibility of Ni Sepharose^™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

특징 및 장점

• High-performance purification of histidine-tagged proteins
• Negligible leakage of Ni²⁺ ions.
• High binding capacity, at least 40 mg/mL medium
• Compatible with a wide range of reducing agents, detergents, denaturants and other additives
• 34 μm bead size for improved resolution

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

Sepharose is a trademark of Cytiva

안전성 정보

• 픽토그램: GHS09
• 신호어: Warning
• 유해 및 위험 성명서: H226
• 예방조치 성명서: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids