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GE17-5110-01

HiTrap® IGM purification

Cytiva 17-5110-01, pack of 5 × 1 mL

동의어(들):

IGM Purification Column

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모든 사진(1)

About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56

ligand

2-mercaptopyridine

포장

pack of 5 × 1 mL

제조업체/상표

Cytiva 17-5110-01

저장 조건

(20% Ethanol)

파라미터

<4 mL/min flow rate
42 psi

베드 크기

7 mm × 25 mm

베드 용적

1 mL

컬럼 I.D.

16 mm

기질

highly cross-linked 6% agarose

평균 부품 크기

34 μm

cleaning

2-13

working range

3-11

저장 온도

2-8°C

일반 설명

HiTrap® IgM Purification HP is a 1 mL column prepacked with Sepharose™ High Performancefor fast and efficient purification of monoclonal IgM.

애플리케이션

HiTrap® IgM Purification HP is prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

특징 및 장점

  • Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
  • Prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance.
  • Recovery of IgM is high with excellent activity retention.
  • The top and bottom frits are manufactured from porous polyethylene
  • Columns are delivered with a stopper on the inlet and a snap-off end on the outle

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

픽토그램

Flame
GHS02

신호어

Warning

유해 및 위험 성명서

H226

Storage Class Code

3 - Flammable liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

문서

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

프로토콜

Performing a Separation with HiTrap® IgM Purifcation HP

How to perform a separation with the HiTrap® IgM Purifcation HP from Cytiva which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from Cytiva.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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