추천 제품
material
polypropylene (PP)
분자량
5000-250000 Da (globular proteins)
포장
pkg of 1 ea
제조업체/상표
Cytiva 17-1195-01
파라미터
<13 mL sample volume
<2.6 mL/min flow rate
0.15 Mpa (22 psi)
베드 용적
320 mL
컬럼 길이 × I.D.
60 cm × 26 mm
Matrix
dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric
기질 활성군
dextran-acrylamide phase
평균 부품 크기
50 μm
cleaning
2-13
작동 범위
3-11
분리 기술
size exclusion (SEC)
일반 설명
HiPrep™ 16/60 Sephacryl® S-200 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl® S-200 is used for separating mid-size proteins including antibodies and serum proteins.
특징 및 장점
- Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
- Gel filtration columns for low to medium pressure AKTA systems.
- Well-established in industrial production.
- The HiPrep™ Sephacryl® series offers reproducible separations over a wide molecular weight range.
- Convenient, prepacked HiPrep™ Sephacryl® columns speed up protein purification.
적합성
All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
for preparative separation of biomolecules of up to 13 mL sample volume
저장 및 안정성
Store at 4 to 30 °C (20% Ethanol)
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
법적 정보
HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
가장 최신 버전 중 하나를 선택하세요:
프로토콜
This page shows how to refold proteins using gel filtration and products from Cytiva.
Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.
관련 콘텐츠
Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.