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주요 문서

GE17-0405-01

HiTrap® Protein G High Performance

Cytiva 17-0405-01, pack of 1 × 5 mL

동의어(들):

Protein G affinity column, Protein purification column

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모든 사진(2)

About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56
가격 및 재고 정보를 현재 이용할 수 없음 고객지원팀으로 연락바랍니다.

ligand

recombinant protein G lacking albumin-binding region

포장

pack of 1 × 5 mL

제조업체/상표

Cytiva 17-0405-01

저장 조건

(20% Ehtanol)
 (20% Ethanol)

파라미터

flow rate
42 psi

베드 크기

16 mm × 25 mm

베드 용적

5 mL

컬럼 I.D.

16 mm

Matrix

highly cross-linked 6% agarose

평균 부품 크기

34 μm

cleaning

2-9

작동 범위

3-9

용량

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
 125 mg binding capacity(human IgG/column)

저장 온도

2-8°C

관련 카테고리

일반 설명

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

특징 및 장점

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

픽토그램

Flame
GHS02

신호어

Warning

유해 및 위험 성명서

H226

Storage Class Code

3 - Flammable liquids

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시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

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문서 라이브러리 방문

Wioleta M Zelek et al.
Immunology, 161(2), 103-113 (2020-06-20)
The implication of complement in multiple diseases over the last 20 years has fuelled interest in developing anti-complement drugs. To date, the focus has been on C5; blocking cleavage of C5 prevents formation of two pro-inflammatory activities, C5a anaphylatoxin and membrane

문서

Automated Antibody Purification with ÄKTA Systems

Designing purification methods for antibodies ensures quality and quantity adequacy for research and industrial purposes.

Performing a Separation with HiTrap® Protein G HP

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

모두 보기

프로토콜

Two-step Purification of Mouse Monoclonal IgG1 Using HiTrap® Protein G HP for the Capture Step

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

General Instructions for Affinity Purification Using HiTrap® Columns

General instructions for affinity purification using HiTrap columns from Cytiva.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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