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G9891

Sigma-Aldrich

β-Glycerophosphate disodium salt hydrate

powder, BioReagent, suitable for cell culture

동의어(들):

BGP, Glycerol 2-phosphate disodium salt hydrate

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About This Item

Linear Formula:
(HOCH2)2CHOP(O)(ONa)2 · xH2O
CAS Number:
154804-51-0
Molecular Weight:
216.04 (anhydrous basis)
EC Number:
212-464-3
MDL number:
MFCD00149082
UNSPSC 코드:
12352200
PubChem Substance ID:
24895383

제품 라인

BioReagent

형태

powder

기술

cell culture | mammalian: suitable

불순물

≤0.20% α-isomer

mp

102-104 °C (lit.)

SMILES string

O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O

InChI

1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2

InChI key

ROPZSVKNEIIIDE-UHFFFAOYSA-L

유사한 제품을 찾으십니까? 방문 제품 비교 안내

애플리케이션

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.
Glycero-2-phosphate is a component of dexamethasone based hormone cocktail used to induce mesechymal stem cell (MSC) differentiation into osteoblasts.
Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

픽토그램

Exclamation mark
GHS07

신호어

Warning

유해 및 위험 성명서

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is this Glycerol 2-phosphate disodium salt hydrate, Product G9891, a cGMP Product?

    Yes. This product is produced under cGMP.

  6. What can I use to solubilize Glycerol 2-phosphate disodium salt hydrate, Product G9891?

    This product is soluble in water at a concentration of 50 mg/mL, yielding a clear, colorless solution.

  7. What concentration of Glycerol 2-phosphate disodium salt hydrate, Product G9891, is used in cell culture?

    Product No. G9891 is tested in cell culture for toxic effects at 0.1 mg/mL. It is used at 10-20 mM for inhibition of phosphatase activity.

  8. Is there an alternative product if Glycerol 2-phosphate disodium salt hydrate, Product G9891, is on backorder?

    Similar products are Product Nos. 50020, G6376 and G1150.

  9. Glycerol 2-phosphate disodium salt hydrate, Product G9891, is "cell culture tested". What does that mean for this product?

    Growth studies are carried through two subculture generations at 0.1 mg/mL. Cells are counted, and growth is plotted as a logarithmic function of time in culture. Seeding efficiencies, doubling time, and final cell densities are determined. During the testing period, cultures are examined microscopically for atypical morphology and evidence of cytotoxicity.

  10. What is the solution stability of Glycerol 2-phosphate disodium salt hydrate, Product G9891?

    β-Glycerophosphate undergoes very slow hydrolysis at 100°C in 1 M hydrochloric acid. The implication is that solutions would be relatively stable at acidic pH.

  11. What is the water content in Glycerol 2-phosphate disodium salt hydrate, Product G9891?

    The water content can vary from lot to lot.  It is typically in the range of 24-31%.  The information can be found on the lot-specific certificate of analysis.

  12. What is Glycerol 2-phosphate disodium salt hydrate, Product G9891, used for?

    β-glycerophosphate is used in cell culture as an inhibitor of alkaline phosphatase. It is routinely used in culture of osteoblast/osteoclast cell lines where the activity of alkaline phosphatase needs to be inhibited (measurement of phosphorylated proteins, etc). A search on PubMed lists over 2500 articles citing "glycerophosphate" and "culture". Most references are for induction of bone lineage cells or for inhibition of phosphatase activity in other types of cell culture.

  13. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Natsumi Watanabe et al.
Human gene therapy, 24(3), 283-294 (2013-01-31)
Hemophilia is an X-linked bleeding disorder, and patients with hemophilia are deficient in a biologically active coagulation factor. This study was designed to combine the efficiency of lentiviral vector transduction techniques with murine adipose tissue-derived stem/stromal cells (mADSCs) as a
Mario-Luca Suvà et al.
Cancer research, 69(5), 1776-1781 (2009-02-12)
Cancer stem cells that display tumor-initiating properties have recently been identified in several distinct types of malignancies, holding promise for more effective therapeutic strategies. However, evidence of such cells in sarcomas, which include some of the most aggressive and therapy-resistant
Monica Echeverry-Rendon et al.
Colloids and surfaces. B, Biointerfaces, 193, 111153-111153 (2020-06-07)
Magnesium (Mg) is a material widely used in industrial applications due to its low weight, ductility, and excellent mechanical properties. For non-permanent implants, Mg is particularly well-suited because of its biodegradability, while its degradation products are not harmful. However, Mg
Lucas R Brun et al.
SpringerPlus, 3, 676-676 (2014-12-19)
The aim of this study was to evaluate the effect of strontium ranelate (SrR) on bone mineral density (BMD) and bone turnover markers after 1 year of treatment. Additionally, the effect of SrR in bisphosphonate-naïve patients (BP-naïve) compared to patients previously
Ludovic Zimmerlin et al.
Tissue engineering. Part A, 17(1-2), 93-106 (2010-08-03)
Adipose-derived stem cells (ASCs) have been proposed to stabilize autologous fat grafts for regenerative therapy, but their safety is unknown in the setting of reconstructive surgery after mastectomy. Both bone marrow mesenchymal stem cells (MSCs) and ASC have been shown
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