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Merck
모든 사진(1)

문서

G1767

Sigma-Aldrich

Glycogen from mussel, Mytilus genus

for DNA precipitations

동의어(들):

DNA and RNA precipitation

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
12352200
NACRES:
NA.52

Grade

for molecular biology

Quality Level

형태

liquid

포장

vial of 1 mL

농도

19-22 mg/mL

저장 온도

−20°C

InChI

1S/C24H42O21/c25-1-5-9(28)11(30)16(35)22(41-5)39-4-8-20(45-23-17(36)12(31)10(29)6(2-26)42-23)14(33)18(37)24(43-8)44-19-7(3-27)40-21(38)15(34)13(19)32/h5-38H,1-4H2/t5-,6-,7-,8-,9-,10-,11+,12+,13-,14-,15-,16-,17-,18-,19-,20-,21+,22+,23-,24-/m1/s1

InChI key

BYSGBSNPRWKUQH-UJDJLXLFSA-N

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일반 설명

Glycogen is a branched polymer of glucose synthesized by animal cells for energy storage and release. It is constructed of predominantly α1→4 glycosidic bonds with branches created through α1→6 glycosidic bonds.
Glycogen is a branched polymer of glucose that coprecipitates nucleic acids during isolation and purification.

애플리케이션

Glycogen from Mytilus genus (Blue mussel) has been used


  • as a coprecipitant in the isolation of RNA from hepatocytes and yeast cells.
  • as a reference standard in calibration curve generation to quantify hepatic glycogen content.
  • as a carrier molecule for DNA and RNA, replacing tRNA or sonicated DNA
  • for the precipitation of DNA in the presence of ammonium acetate and ethanol or isopropanol
  • for recovery of tRNA, yeast RNA, sonicated DNA and oligonucleotides as short as 8 base pairs

생화학적/생리학적 작용

Glycogen levels in Mytilus genus shows variation during seasonal changes. It is a crucial stored energy reserve for Mussels and is utilized during gametogenesis. Glycogen levels dictate the reproductive capacity and development of mussels larva. Glycogen aids in the coprecipitation of nucleic acids during isolation and purification.

특징 및 장점

  • product provided is 1 mL total volume
  • precipitates even small amounts of DNA and RNA present in dilute solutions
  • does not interfere with OD 260/280 readings

원리

Glycogen is an inert carrier that traps nucleic acids. It forms a precipitate with DNA/RNA while being insoluble in ethanol solution. Due to the manufacturing process, there is low risk of contaminating nucleic acids in the glycogen. This product is tested for DNase, RNase, and NICKase, but not nucleic acids.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Regulation of gluconeogenesis by Kruppel-like factor 15
Gray S, et al.
Cell Metabolism, 5(4), 305-312 (2007)
Energy storage and reproduction in mussels, Mytilus galloprovincialis: the influence of diet quality
Fearman JA, et al.
Journal of shellfish research., 28(2), 305-312 (2009)
Tri-methylation of histone H3 lysine 4 facilitates gene expression in ageing cells
Cruz C, et al.
eLife, 7, e34081-e34081 (2018)
Biomass composition of blue mussels, Mytilus edulis, is affected by living site and species of ingested microalgae
Pleissner D, et al.
ISRN zoology, 2012 (2012)
Sergey V Prykhozhij et al.
Disease models & mechanisms, 10(6), 811-822 (2017-03-11)
Clustered regularly interspaced palindromic repeats (CRISPR)/Cas-based adaptive immunity against pathogens in bacteria has been adapted for genome editing and applied in zebrafish (Danio rerio) to generate frameshift mutations in protein-coding genes. Although there are methods to detect, quantify and sequence

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