F4229

Monoclonal Anti-Fas (CD95/Apo-1)−FITC antibody produced in mouse

clone DX2, purified immunoglobulin, buffered aqueous solution

• 동의어(들): Monoclonal Anti-Fas, Anti-Apo-1, Anti-CD95
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: mouse
• 결합: FITC conjugate
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: DX2, monoclonal
• 양식: buffered aqueous solution
• 종 반응성: human
• 기술: flow cytometry: 1:50 using Cultured human Burkitt′s lymphoma Raji cells
• 동형: IgG1
• UniProt 수납 번호: P25445
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 유전자 정보: human ... FAS(355)

설명

일반 설명

Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. The most well studied receptors are CD95/Fas/Apo-1 (apoptosis inducing protein 1) and tumor necrosis factor receptor 1 (TNFR1). Apoptosis mediated by either of these results in activation of the caspases. However, Fas-mediated death occurs much more rapidly than that triggered by TNFR1. Human Fas/CD95/Apo-1 is a single transmembrane glycoprotein receptor (325 amino acids, 45-48 kDa).

특이성

Reacts specifically with the functional epitope of human Fas (CD95/Apo-1) antigen. By immunoblotting, the clone recognizes denatured, non-reduced recombinant human Fas (amino acid residues 1-173). The antibody is reactive in flow cytometry, and may be reactive in the induction of apoptosis.

면역원

murine L cells transfected with a human Fas/CD95 cDNA.

애플리케이션

Monoclonal Anti-Fas (CD95/Apo-1)-FITC antibody is suitable for flow cytometry at a dilution of 1:50 using cultured human Burkitt′s lymphoma Raji cells.

생화학적/생리학적 작용

APO-1/Fas(CD95) comprises of a death domain (DD) within the cytoplasmic region which triggers apoptosis upon binding of their cognate ligands. Once it is activated, APO-1/Fas(CD95) further aggregates its intracellular death domains which leads to the recruitment of two key signaling proteins followed by the formation of death-inducing signaling complex. These complex crosslinks through its C-terminal DD with APO-1/Fas receptors and engage caspase-8 via its N-terminal death effector domain (DED) to the DISC.[1]

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.