F4165

Ficin from fig tree latex

powder, ≥0.1 unit/mg solid

• 동의어(들): Debricin, Ficain, higueroxyl delabarre
• CAS Number: 9001-33-6
• 효소 위원회 번호: 3.4.22.3 (BRENDA, IUBMB)
• EC Number: 232-599-1
• MDL number: MFCD00081597
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: fig tree (latex)
• 형태: powder
• 특이 활성도: ≥0.1 unit/mg solid
• 분자량: 23.8 kDa
• 저장 온도: −20°C

설명

일반 설명

Extinction Coefficient: E^1% = 21.0 (280 nm)
pI: 9.0

생화학적/생리학적 작용

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported K_m for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)₂ fragments from mouse IgG1.

단위 정의

One unit will produce a ΔA₂₈₀ of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).

The enzyme is soluble in 1 M potassium phosphate buffer, pH 7.0 (0.25 mg/ml), yielding a clear solution.

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves