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Merck
모든 사진(1)

문서

F3665

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O111:B4

FITC conjugate

동의어(들):

LPS

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About This Item

EC Number:
MDL number:
UNSPSC 코드:
12352201
NACRES:
NA.25

생물학적 소스

Escherichia coli (O111:B4)

Quality Level

결합

FITC conjugate

형태

lyophilized powder

라벨링 범위

2-10 μg FITC per mg LPS

색상

yellow to orange

solubility

H2O: soluble 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow

저장 온도

2-8°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

애플리케이션

FITC-Lipopolysaccharides (FITC-LPS) may be used to study processes such as the movement/transport of LPS across tissue barriers such as the colonic epithelium and cellular binding and internalization of lipopolysaccharides with fluorescence-based assay systems. It may be used to evaluate, qualify and quantify lipopolysaccharides (LPS) removal reagents and procedures. FITC-LPS conjugate may be used for the detection of LPA binding proteins and motifs.
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

생화학적/생리학적 작용

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

제조 메모

The product is soluble in water (5 mg/ml) yielding a hazy, yellow to orange solution. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution. For cell culture use, LPS should be reconstituted by adding 1 ml of sterile balanced salt solution or cell culture medium to a vial (1 mg) and swirling gently until the powder dissolves. Solutions can be further diluted to the desired working concentration with additional sterile balanced salt solutions or cell culture media.

기타 정보

To gain a comprehensive understanding of our extensive range of lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

픽토그램

Skull and crossbones

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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문서

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

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