F2006

Human Fibronectin

from human plasma, powder, suitable for cell culture

• 동의어(들): Fibronectin
• EC Number: 289-149-2
• MDL number: MFCD00131062
• UNSPSC 코드: 12352202
• eCl@ss: 42020141
• NACRES: NA.75

속성

• product name: Fibronectin human plasma, lyophilized powder, BioReagent, suitable for cell culture
• 생물학적 소스: human plasma
• Quality Level: 200
• 무균: sterile
• 제품 라인: BioReagent
• 분석: ≥85% (SDS-PAGE)
• 형태: lyophilized powder
• 분자량: 450 kDa
• 포장: pkg of 5 x 5 mg; pkg of 1 mg; pkg of 2 mg; pkg of 5 mg
• 농도: >1 mg/mL protein
• 기술: cell culture | mammalian: suitable
• 표면 범위: 1‑5 μg/cm²
• 불순물: HBSAG, none detected; HCV, none detected; HIV-1/HIV-2, none detected
• solubility: H₂O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)
• NCBI 수납 번호: NM_212482.4
• UniProt 수납 번호: P02751
• 결합 특이성: Peptide Source: Collagen
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: human ... FN1(2335)

설명

일반 설명

Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous in nature and form functional unit arranged linearly on two subunit arms. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of fibronectin protein due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.

애플리케이션

Fibronectin from human plasma is used for the following applications:

• Used in cell seeding and neuronal differentiation (as one of the components in expansion medium for the re-suspension of CBSCs (Cord blood stem cells) and is also used in differentiation medium)
• Used in cell culture (the culture chamber was coated with fibronectin for the expression of HEK293 cells)
• In-vitro fibronectin (FN) pulldown assays
• Protein specificity
• Used in cell migration study

생화학적/생리학적 작용

Fibronectin (FN) plays an essential role in multiple biological functions such as, angiogenesis, cell migration cell-to-cell adhesion, cell-to-basement-membrane attachment, clot stabilization, embryogenesis, differentiation, wound healing, as well as binding of pathogen to mammalian cells and extracellular matrix. Studies in Chinese Han population show that mutations in this gene might be linked with susceptibility to knee osteoarthritis (OA). Splice variant extra domain-B (EDB) of FN is a hallmark of tumor angiogenesis, and with increased matrix stiffness there is an increase in the production of this isoform by endothelial cells. In colorectal cancer (CRC), EDA is linked with metastasis, vasculogenesis and tumorigenesis and promotes epithelial-mesenchymal transition.

주의사항

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

제조 메모

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B, hepatitis C and syphilis. In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)