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추천 제품
태그
FLAG® tagged
Grade
Molecular Biology
for molecular biology
양식
buffered aqueous solution
배송 상태
dry ice
저장 온도
−20°C
일반 설명
The pFLAG-CTS™ Expression Vector is a 5.3 kb E. coli expression vector used for cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG® fusion protein. The FLAG epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products.
C-terminal FLAG fusion proteins may be purified using Monoclonal ANTI-FLAG M2, Catalog Number F3165, and ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.
The pFLAG-CTS-BAP Control Plasmid is a 6.7 kb E. coli plasmid used for efficient and controlled periplasmic expression of C-terminal FLAG-BAP fusion protein.
Vector Maps and Sequences
C-terminal FLAG fusion proteins may be purified using Monoclonal ANTI-FLAG M2, Catalog Number F3165, and ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.
The pFLAG-CTS-BAP Control Plasmid is a 6.7 kb E. coli plasmid used for efficient and controlled periplasmic expression of C-terminal FLAG-BAP fusion protein.
Vector Maps and Sequences
애플리케이션
The pFLAG-CTS™ Expression Vector is suitable for cloning and expression of C-terminal FLAG® fusion proteins in E. coli.
성분
- pFLAG-CTS™ Expression Vector 10 μg (E5269) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
- pFLAG-CTS™-BAP Control Plasmid 1 μg (P7707) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
원리
The promoter-regulatory region of the strong tac promoter (a hybrid of the trp and lac promoters from E.coli) drives transcription of ORF-FLAG fusion constructs. Control of transcription is regulated by the presence of the lacO sequences and inclusion of the lac repressor gene (lacI) on the plasmid.
법적 정보
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CTS is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
가장 최신 버전 중 하나를 선택하세요:
G A Smith et al.
Molecular microbiology, 17(5), 945-951 (1995-09-01)
Listeria monocytogenes is a Gram-positive facultative intracytoplasmic bacterial pathogen that exhibits rapid actin-based motility in eukaryotic cells and in cell-free cytoplasmic extracts. The protein product of the actA gene is required for bacterial movement and is normally expressed in a
Saadet Albayrak Guralp et al.
PloS one, 8(3), e59305-e59305 (2013-03-26)
Antimicrobial peptides (AMPs) belong to a class of natural microbicidal molecules that have been receiving great attention for their lower propensity for inducing drug resistance, hence, their potential as alternative drugs to conventional antibiotics. By generating AMP libraries, one can
H A de Boer et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(1), 21-25 (1983-01-01)
Two hybrid promoters that are functional in Escherichia coli have been constructed. These hybrid promoters, tacI and tacII, were derived from sequences of the trp and the lac UV5 promoters. In the first hybrid promoter (tacI), the DNA upstream of
Montarop Yamabhai et al.
Journal of biotechnology, 133(1), 50-57 (2007-10-24)
Bacillus spp. are Gram-positive bacteria that secrete a large number of extracellular proteins of industrial relevance. In this report, three Bacillus extracellular hydrolytic enzymes, i.e., alpha-amylase, mannanase and chitinase, were cloned and over-expressed in Gram-negative Escherichia coli. We found that
Parisa Asvadi et al.
Journal of molecular recognition : JMR, 15(5), 321-330 (2002-11-26)
In an attempt to generate recombinant anti-D reagents for possible diagnostic and therapeutic use we cloned the genes encoding the variable (V) domains of a human anti-D antibody secreted by the lymphoblastoid cell line BTSN4. A single-chain Fv (scFv) fragment
관련 콘텐츠
Bacterial Expression Vectors: tac Promoter System
활성 필터
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