Endotoxin Removal solution has been used to remove lipopolysaccharide (LPS) from Baker′s yeast RNA.[1]
생화학적/생리학적 작용
The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase.
수량
1 volume treats 5 volumes of DNA solution (≤1mg/ml)
Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin fibroblasts, primary human melanoma cells)