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Merck
모든 사진(1)

문서

D9538

Sigma-Aldrich

N,N′-(1,2-Dihydroxyethylene)bis-acrylamide

suitable for electrophoresis

동의어(들):

DHEBA, N,N′-Bisacryloyl-1,2-dihydroxy-1,2-ethylenediamine

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About This Item

실험식(Hill 표기법):
C8H12N2O4
CAS Number:
Molecular Weight:
200.19
Beilstein:
1941875
EC Number:
MDL number:
UNSPSC 코드:
12162002

기술

electrophoresis: suitable

전도도

<60 μmho (2% solution)

저장 온도

2-8°C

SMILES string

OC(NC(=O)C=C)C(O)NC(=O)C=C

InChI

1S/C8H12N2O4/c1-3-5(11)9-7(13)8(14)10-6(12)4-2/h3-4,7-8,13-14H,1-2H2,(H,9,11)(H,10,12)

InChI key

ZMLXKXHICXTSDM-UHFFFAOYSA-N

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애플리케이션

Reversible cross-linker for polyacrylamide gels that produces a gel, which is soluble in dilute (2%) periodic acid at room temperature for 1-2 hr. Gel solubilization is useful when recovering protein from polyacrylamide gels.

분석 메모

Tested for use as a cross-linking agent for recovery of separated biomolecules.

픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves


시험 성적서(COA)

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문서 라이브러리 방문

U Neumann et al.
Analytical biochemistry, 206(1), 1-5 (1992-10-01)
A simple and rapid method for the quantitation of proteins separated either by sodium dodecyl sulfate-electrophoresis or by isoelectric focusing in slab gels is presented. The method is based on the solubility of polyacrylamide gels crosslinked with N, N'-1, 2-dihydroxyethylenebisacrylamide
A method for the quantitative determination of protein incorporated in solubilized polyacrylamide gels.
J Tas et al.
Analytical biochemistry, 100(2), 264-270 (1979-12-01)
M Galvani et al.
Electrophoresis, 21(17), 3684-3692 (2001-03-29)
A number of cross-linkers that are commonly used in polyacrylamide gels have been incubated with bovine beta-lactoglobulin B and the resulting reaction mixtures were examined by matrix assisted laser desorption/ionization-mass spectrometry. At concentrations of 0.1, 1, and 20 mM of
Polyacrylamide gels with modified cross-linkages.
P B O'Connell et al.
Analytical biochemistry, 76(l), 63-73 (1976-11-01)
M Burow et al.
Biochemical and biophysical research communications, 227(2), 419-422 (1996-10-14)
A phosphate buffer containing a mixture of glucose oxidase, acrylic acid derivatives, N,N'-1,2 dihydroxy-ethylene-bis(acrylamide), N,N'-(methylene)-bisacrylamide and surface-modified silica was radically polymerized with (NH4)2S2O8. The polymer formed a thin layer around the silica beads. After sieving of these polymer particles, the

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