D6944

Anti-Dopamine Transporter (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-DAT, Anti-DAT1, Anti-SLC6A3, Anti-Solute carrier family 6, member 3
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~50 kDa
• 종 반응성: mouse (predicted), rat, human
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• 농도: ~1.0 mg/mL
• 기술: immunofluorescence: 5-10 μg/mL using SH-SY-5Y cells; western blot: 0.5-1 μg/mL using rat brain tissue extract
• UniProt 수납 번호: Q01959
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SLC6A3(6531) ; mouse ... Slc6a3(13162) ; rat ... Slc6a3(24898)

설명

일반 설명

Dopamine transporter belongs to a family of biogenic amine transporters that include the norepinephrine, serotonin (SERT), and vesicular monoamine transporters. These transporters are members of a larger Na⁺/Cl^--dependent neurotransmitter transporter family.

Solute carrier family 6 member 3 (SLC6A3) is mapped to human chromosome 5p15.33. It is also known as the dopamine transporter (DAT) and has a zinc binding site and 12 transmembrane region.

면역원

synthetic peptide corresponding to amino acids 42-59 of rat dopamine transporter conjugated to KLH. This sequence is highly conserved in mouse DAT (single amino acid substitution) and has lower homology (72% identity) to human DAT.

애플리케이션

Anti-Dopamine Transporter antibody produced in rabbit is suitable for western blot analysis at a working concentration of 0.5-1μg/mL using rat brain extract (cytosolic S1 fraction) and HEK-293T cells expressing human DAT1. It was used as a primary antibody in western analysis for dopamine transporter in several studies.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

생화학적/생리학적 작용

DAT regulates dopaminergic-neurotransmission. It is localized on presynaptic nerve terminals and mediates the active reuptake of dopamine from the synapse, thus terminating the neurotransmitter signal. It is the primary site for the action of neurotoxins, such as MPTP, and the drugs of abuse, cocaine and amphetamine. It has been implicated in a number of dopamine-related disorders, including attention deficit hyperactivity disorder (ADHD), bipolar disorder, clinical depression, and alcoholism. DAT may also have a role in selective vulnerability of nigrostriatal dopaminergic neurons in Parkinson′s disease as it can transport dopamine neurotoxins.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)