D5167

Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse

clone 70.1, ascites fluid

• 동의어(들): Anti-CILD1, Anti-DIC1, Anti-ICS1, Anti-PCD
• MDL number: MFCD02262824
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: 70.1, monoclonal
• 포함: 15 mM sodium azide
• 종 반응성: marsupial, hamster, human, Indian muntjac (Muntiacus muntjac vaginalis), chicken, rat, frog
• 기술: immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 1:2,000 using a chick brain extract
• 동형: IgM
• UniProt 수납 번호: Q9UI46
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... DNAI1(27019) , DNAI2(64446) ; rat ... Dnai2(360654)

설명

일반 설명

Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus. Monoclonal anti-dynein (Intermediate Chain) antibody can be used for localization of dynein intermediate chain using different immunochemical assays like ELISA, immunoblotting and dot blot. Mouse anti- dynein (Intermediate Chain) antibody reacts specifically with dynein of frog, hamster (CHO cell line), chicken, rat, marsupial (rat kangaroo, PtK1 cell line), Indian muntjac (Muntiacus muntjac vaginalis) and human (HeLa,MCF-7).

Monoclonal anti-Dynein (Intermediate Chain) (mouse IgM isotype) is derived from the 70.1 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cytoplasmic dynein purified from chick brain. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (approximately 450 kDa), three intermediate chains (70- 125 kDa), and at least four light chains (15-25 kDa). Dynein intermediate chain (ICs) is a component of dynein multisubunit. Dynein intermediate chain interacts with dynactin through its N-terminal region.

면역원

cytoplasmic dynein from chicken embryo brain

애플리케이션

Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse has been used in:

• perturbation assays
• western blotting
• immunofluorescence microscopy
• enzyme linked immunosorbent assay (ELISA)
• dot blot
• immunocytochemistry
• immunopurification

생화학적/생리학적 작용

Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable