MAB1618
Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1
clone 74.1, Chemicon®, from mouse
동의어(들):
Cytoplasmic dynein 1 intermediate chain 1, Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1, Cytoplasmic dynein 1 intermediate chain 2, Cytoplasmic dynein intermediate chain 2, Dynein intermediate chain 2, cytosoli
About This Item
추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
74.1, monoclonal
종 반응성
Xenopus, mouse, fish, rat, human, sheep, Drosophila, bovine
반응하면 안 됨
squid
제조업체/상표
Chemicon®
기술
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG2b
NCBI 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... DYNC1I1(1780)
일반 설명
특이성
애플리케이션
1:50-1:100 dilution from a previous lot was used. Reacts with cultured MDCK cells, NRR cells, N1E cells, and PTK-1 cells.
Immunoprecipitation:
10 μL of a previous lot per 0.5 gm tissue was used. The monoclonal cleanly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530 kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates.
Immunoblotting:
1:1,000-1:5000. Reacts with cultured MDCK cells, PC-12 cells, N1E cells, neurons, glia and other cultured cells.
Optimal working dilutions must be determined by end user.
품질
Western Blot Analysis:
1:500 dilution of this lot detected DYNEIN on 10 μg of A431 lysates.
표적 설명
물리적 형태
분석 메모
HeLa cells, A431 cell lysate
기타 정보
법적 정보
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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