Unfortunately, no data for Product D4008's use with flow cytometry application is determined. However, several academic publications associated with this product's use in flow cytometry experiments may provide details that can assist with the experimental design. Please see the link to those publications below:
https://scholar.google.com/scholar?hl=en&as_sdt=0%2C26&q=%22Dansylcadaverine%22+%22D4008%22+%22flow+cytometry%22&btnG=
D4008
Dansylcadaverine
≥97% (TLC)
동의어(들):
N-(5-Aminopentyl)-5-dimethylaminonaphthalene-1-sulfonamide, N-(Dimethylaminonaphthalenesulfonyl)-1,5-pentanediamine, Monodansyl cadaverine
로그인조직 및 계약 가격 보기
모든 사진(1)
크기 선택
보기 변경
100 MG
₩84,042
250 MG
₩166,054
1 G
₩425,488
About This Item
실험식(Hill 표기법):
C17H25N3O2S
CAS Number:
Molecular Weight:
335.46
Beilstein:
2951070
EC Number:
MDL number:
UNSPSC 코드:
12352108
PubChem Substance ID:
NACRES:
NA.32
추천 제품
일반 설명
Dansylcadaverine, also called as monodansylcadaverine (MDC), is a lysosomotropic agent. It is present in acidic and lipid rich regions. Dansylcadaverine also acts as a solvent polarity probe.[1] It inhibits vesicular transport and decreases the rotavirus sequestering in L cells, when pre-treated.[2]
애플리케이션
Dansylcadaverine in methanol has a reported λex of 335 nm and λem of 512 nm. It has been used:
- as an endocytosis inhibitor in human proximal tubular HK-2 cells,(62) mouse J774A.1 macrophages and human A549 epithelial cells(63)
- to label autophagic vacuoles in cardiomyocytes for fluorescence microscopy studies(64)
- in monodansylcadaverine (MDC) staining of fibroblasts in Ankylosing spondylitis (AS) samples(65)
생화학적/생리학적 작용
The autofluorescent dye dansylcadaverine, or monodansylcadaverine (MDC), is commonly used to monitor autophagy. MDC preferentially accumulates in autophagic vacuoles due to a combination of ion trapping and specific interactions with membrane lipids.
포장
Bottomless glass bottle. Contents are inside inserted fused cone.
air sensitive, handled under inert gas
기타 정보
Solubility of dansylcadaverine is tested in methanol (10 mg/mL). It has also been reported that dansylcadaverine is soluble in DMSO and acetic acid.
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
dust mask type N95 (US), Eyeshields, Gloves
이미 열람한 고객
Fluorescence properties and staining behavior of monodansylpentane, a structural homologue of the lysosomotropic agent monodansylcadaverine
Niemann A, et al.
The Journal of Histochemistry and Cytochemistry, 49(2), 177-185 (2001)
Dansylcadaverine and cytochalasin D enhance rotavirus infection of murine L cells
Bass DM, et al.
Virology, 212(2), 429-437 (1995)
Melissa S Hillwig et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(3), 1093-1098 (2011-01-05)
RNase T2 enzymes are conserved in most eukaryotic genomes, and expression patterns and phylogenetic analyses suggest that they may carry out an important housekeeping role. However, the nature of this role has been elusive. Here we show that RNS2, an
Raúl A Almeida et al.
Veterinary research communications, 35(2), 89-101 (2011-01-06)
Research on mastitis in dairy cows caused by Escherichia coli has reported the emergence of strains capable of inducing chronic mastitis and that these strains adhered to and internalized into bovine mammary epithelial cells better than strains of E. coli
Shih T Khew et al.
Biomaterials, 31(16), 4600-4608 (2010-03-13)
Transglutaminase (TGase)-modified proteins are commonly observed in a wide range of biological systems. Therefore, the identification of TGase substrates and respective consensus sites may contribute to a better understanding of the physiological role of TGase. In this study, we identified
-
Can I use Dansylcadaverine D4008 for detection of autophagy using flow cytometry? if Yes which laser should we use to detect the MDC signal? Di you have any recommendations regarding the final concentration of MDC for detection of autophagy in lymphocyte?
1 답변-
도움이 되었습니까?
-
-
Can Dansylcadaverine be use as a marker of autophagic vacuoles?
1 답변-
Dansylcadaverine is a lysosomotropic compound that can be used for the identification of autophagic vacuoles. Please refer to the literature for application-specific protocols.
도움이 되었습니까?
-
-
What is Product D4008, Dansylcadaverine, soluble in?
1 답변-
We test the solubility of this product in acetic acid at a concentration of 50 mg/mL, with the application of heat and sonication. It has also been reported as being soluble in methanol and DMSO. We have not validated this claim in our labs.
도움이 되었습니까?
-
-
What is the solution stability of Product D4008, Dansylcadaverine?
1 답변-
According to one source a stock solution of monodansylcadaverine was prepared in methanol at 50 mM and stored frozen at -20°C. No time frame for long-term solution stability was given.
도움이 되었습니까?
-
-
What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
도움이 되었습니까?
-
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
