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Merck
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주요 문서

CLLS1011

Sigma-Aldrich

SW48 Cells BAX/BAK (-/-,-/-)

Human female colon, colorectal adenocarcinoma

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About This Item

UNSPSC 코드:
41106514

제품명

SW48 Cells BAX/BAK (-/-,-/-),

생물학적 소스

human female colon (Source Disease: Colorectal adenocarcinoma)

OMIM 수납 번호

저장 온도

−196°C

유전자 정보

human ... BAK(578) , BAX(581)

일반 설명

This product corresponds to ATCC Cat. No. CCL-231

애플리케이션

Media Renewal changes one to two times per week
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:2 to 1:6
The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, Sodium pyruvate, Cat. No. S8636, at a final concentration of 1 mM, and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
The cell freezing medium is DMSO 1X (Cat. No. C6164)

특징 및 장점

These SW48 cells are adherent, with a doubling time of approx. 35 hours.
Zinc finger nuclease (ZFN) double knock out on chromosome 19q13.3-13.4 / 6p21.3

품질

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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제품 번호
설명
SDS

  • SW48 Cells BAX/BAK (-/-,-/-) 1 vial

  • SW48 Control Cells 1 vial

Storage Class Code

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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