CLLS1003

DLD1 Cells PIK3CA +/-

human male colon (Source Disease: Colorectal adenocarcinoma)

• UNSPSC 코드: 41106514
• NACRES: NA.81

속성

• product name: DLD1 Cells PIK3CA +/-,
• 생물학적 소스: human male colon (Source Disease: Colorectal adenocarcinoma)
• OMIM 수납 번호: 171834
• 저장 온도: −196°C
• 유전자 정보: human ... PIK3CA(5290)

설명

일반 설명

DLD1 Cells PIK3CA +/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.

애플리케이션

Media Renewal changes two to three times per week

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3 to 1:10

The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.

The cell freezing medium is DMSO 1X (Cat. No. C6164)

특징 및 장점

These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.

Zinc finger nuclease (ZFN) knock out on chromosome 3q23.3

품질

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

키트 구성품 역시 별도로 이용 가능함

• DLD1 Control Cells 1 vial
• DLD1 Cells PIK3CA +/- 1 vial

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable