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Merck
모든 사진(1)

문서

CG201

Sigma-Aldrich

cGMP Enzyme Immunoassay Kit

sufficient for 96 assays

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.32

사용

sufficient for 96 assays

Quality Level

기술

ELISA: suitable

배송 상태

wet ice

저장 온도

−20°C

유전자 정보

human ... PRKG1(5592)

일반 설명

cGMP Enzyme Immunoassay Kit is non-radioactive, competitive immunoassay for the quantitation of cyclic guanine monophosphate (cGMP) in biological fluids (serum, plasma, urine and saliva), tissue culture media samples or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The immunoassay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.

애플리케이션

Non-radioactive, competitive immunoassay for the quantitation of cGMP in biological fluids (serum, plasma, urine and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.
cGMP Enzyme Immunoassay Kit has been used for the quantitative determination of cyclic guanine monophosphate (cGMP) concentration in parasite tissues and mouse aortic artery homogenates. It has also been used to assess the guanylate cyclase activity.

키트 구성품 전용

제품 번호
설명

  • Acetic Anhydride 1 x 1

  • Assay Buffer 2 1 x 30

  • cGMP-Alkaline Phosphatase Conjugate 1 x 5

  • cGMP Assay Layout Sheet 1 x 1

  • cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5

  • 5 Cycle Logit-Log Paper 1 x 1

  • Cyclic GMP Standard 1 x 0.5

  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea

  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20

  • Plate Sealer 1 ea

  • Stop Solution 1 x 5

  • Triethylamine 1 x 2

  • Wash Buffer Concentrate 1 x 30

모두 보기 (13)

픽토그램

FlameSkull and crossbonesCorrosion

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1A - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

3 - Flammable liquids

Flash Point (°F)

12.2 °F - closed cup

Flash Point (°C)

-11 °C - closed cup


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Identification of a novel Arabidopsis thaliana nitric oxide-binding molecule with guanylate cyclase activity in vitro
Mulaudzi T, et al.
Febs Letters, 585(17), 2693-2697 (2011)
Genistein from Flemingia vestita (Fabaceae) enhances NO and its mediator (cGMP) production in a cestode parasite, Raillietina echinobothrida
Das B, et al.
Parasitoloty, 134(10), 1457-1463 (2007)
Inhibition of MPO (Myeloperoxidase) Attenuates Endothelial Dysfunction in Mouse Models of Vascular Inflammation and Atherosclerosis
Cheng D, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 39(7), 1448-1457 (2019)
David Cheng et al.
Arteriosclerosis, thrombosis, and vascular biology, 39(7), 1448-1457 (2019-05-03)
Objective- Inflammation-driven endothelial dysfunction initiates and contributes to the progression of atherosclerosis, and MPO (myeloperoxidase) has been implicated as a potential culprit. On release by circulating phagocytes, MPO is thought to contribute to endothelial dysfunction by limiting NO bioavailability via
Sara Falvo et al.
Animals : an open access journal from MDPI, 11(6) (2021-07-03)
The quail Coturnix coturnix is a seasonal breeding species, with the annual reproductive cycle of its testes comprising an activation phase and a regression phase. Our previous results have proven that the testicular levels of both 17β-estradiol (E2) and androgens

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