LB185

98062318, human blood, Lymphoblastoid

• UNSPSC 코드: 41106514

속성

• 제품명: LB185, 98062318
• 생물학적 소스: human blood
• 성장 모드: Suspension
• 핵형: Not specified
• 형태학: Lymphoblastoid
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human lymphoblastoid, normal,EBV-transformed

세포주 설명

The lymphocyte cell line LB185 has been established using mononuclear cells from peripheral blood of a 56 year old normal male. T cells from the same sample have been used as controls for cellular radiosensitivity studies. Cells have been subsequently transformed with EBV. Fibroblasts have been derived from the same individual and were designated 153BR (Sigma Catalogue number. 90011808). This has been confirmed by STR profiling at ECACC. Cells are lymphhoblastoid with floating aggregates present.

애플리케이션

Mutation studies

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 9
D5S818: 10,13
D7S820: 11
THO1: 8,9
TPOX: 8,10
vWA: 17,18

배양 배지

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

계대배양 정규 작업

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

기타 정보

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